Determination and metabolism of dithiol chelating agents. IV. Urinary excretion of meso-2,3-dimercaptosuccinic acid and mercaptosuccinic acid in rabbits given meso-2,3-dimercaptosuccinic acid.
Determination and metabolism of dithiol chelating agents. IV. Urinary excretion of meso-2,3-dimercaptosuccinic acid and mercaptosuccinic acid in rabbits given meso-2,3-dimercaptosuccinic acid.
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二硫醇螯合剂的测定和代谢。
DOI：
10.1016/0006-2952(89)90261-x
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发表时间：
1989
影响因子：
5.8
通讯作者：
Aposhian,HV
中科院分区：
文献类型：
--
作者：
Maiorino,RM;Aposhian,HV
水溶性二巯基螯合剂间二巯基丁二酸（DMSA）和2，3-二巯基丙磺酸（DMPS）在铅、砷、汞中毒的治疗中越来越受到重视。然而，关于它们的代谢转化的数据很少。雄性家兔肌肉注射DMSA（0.20 mmol/kg），并在6小时内收集尿液。Monobromobimane衍生化，HPLC分离，荧光检测，沿着与[U-14 C]DMSA数据，证明了在尿液中发现的总14 C分布为73%未改变的DMSA，7%巯基琥珀酸和6%和14%的两个未知数。电解还原处理的尿液没有增加尿中的DMSA含量，表明氧化生物转化是不是一个主要的途径DMSA在兔。后者的结果与DMPS在兔中的结果显著不同。
The water-soluble dithiol chelating agentsmeso-2,3-dimercaptosuccinic acid (DMSA) and 2,3-dimercaptopropane-l-sulfonic acid (DMPS) are becoming of increasing importance for the treatment of lead, arsenic and mercury poisoning. There is, however, a paucity of data about their metabolic transformation. Male rabbits were given DMSA (0.20 mmol/kg) i.m., and urine was collected over a 6-hr period. Monobromobimane derivatization, HPLC separation, and fluorescence detection, along with [U-14C]DMSA data, demonstrated that the total14C found in the urine was distributed as 73% unaltered DMSA, 7% mercaptosuccinic acid and 6 and 14% of two unknowns. Electrolytic reductive treatment of the urine did not increase the urinary content of DMSA, indicating that oxidative biotransformation is not a major pathway for DMSA in the rabbit. This latter result is strikingly different from that for DMPS in rabbit.