Air pollution exposure during critical time periods in gestation and alterations in cord blood lymphocyte distribution: a cohort of livebirths.
Air pollution exposure during critical time periods in gestation and alterations in cord blood lymphocyte distribution: a cohort of livebirths.
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DOI：
10.1186/1476-069x-9-46
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发表时间：
2010-08-02
期刊：
影响因子：
--
通讯作者：
Hertz-Picciotto I
中科院分区：
文献类型：
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作者：
Herr CE;Dostal M;Ghosh R;Ashwood P;Lipsett M;Pinkerton KE;Sram R;Hertz-Picciotto I
有毒暴露已被证明会影响怀孕期间免疫系统的成熟。本研究调查了脐带血淋巴细胞比例与母亲在妊娠每个月暴露于空气污染之间的关系。使用FACSort流式细胞仪分析脐带血，以确定T淋巴细胞（CD3+细胞及其亚群，CD4+和CD8+）， B淋巴细胞（CD19+）和自然杀伤（NK）细胞的比例。采用固定监测仪测量环境空气中12种多环芳烃（PAH）和直径< 2.5微米颗粒物（PM2.5）的浓度。这些污染物的算术平均值，计算每个妊娠月，被用作暴露指标。协变量数据来自医疗记录和问卷调查。拟合多变量线性回归模型来估计每月PAH或PM2.5与脐带血淋巴细胞之间的关系，调整出生年份和居住地区，并在进一步的模型中调整妊娠季节和先前活产的数量。调整后的模型显示，妊娠早期多环芳烃或PM2.5与脐带血CD3+和CD4+淋巴细胞百分比升高、CD19+和NK细胞百分比降低之间存在显著关联。相反，妊娠后期暴露与CD3+和CD4+含量降低以及CD19+和NK细胞含量增加有关。在妊娠中期，淋巴细胞分布的改变与空气污染暴露之间没有显著的联系。环境空气中的多环芳烃和PM2.5可能通过改变脐带血淋巴细胞分布影响胎儿免疫发育。在妊娠早期和妊娠晚期暴露的相关性似乎有所不同。
Toxic exposures have been shown to influence maturation of the immune system during gestation. This study investigates the association between cord blood lymphocyte proportions and maternal exposure to air pollution during each gestational month. Cord blood was analyzed using a FACSort flow cytometer to determine proportions of T lymphocytes (CD3+ cells and their subsets, CD4+ and CD8+), B lymphocytes (CD19+) and natural killer (NK) cells. Ambient air concentrations of 12 polycyclic aromatic hydrocarbons (PAH) and particulate matter < 2.5 micrometer in diameter (PM2.5) were measured using fixed site monitors. Arithmetic means of these pollutants, calculated for each gestational month, were used as exposure metrics. Data on covariates were obtained from medical records and questionnaires. Multivariable linear regression models were fitted to estimate associations between monthly PAH or PM2.5 and cord blood lymphocytes, adjusting for year of birth and district of residence and, in further models, gestational season and number of prior live births. The adjusted models show significant associations between PAHs or PM2.5 during early gestation and increases in CD3+ and CD4+ lymphocytes percentages and decreases in CD19+ and NK cell percentages in cord blood. In contrast, exposures during late gestation were associated with decreases in CD3+ and CD4+ fractions and increases in CD19+ and NK cell fractions. There was no significant association between alterations in lymphocyte distribution and air pollution exposure during the mid gestation. PAHs and PM2.5 in ambient air may influence fetal immune development via shifts in cord blood lymphocytes distributions. Associations appear to differ by exposure in early versus late gestation.