Mapping of left ventricle wall thickness in mice using 11.7-T magnetic resonance imaging
Mapping of left ventricle wall thickness in mice using 11.7-T magnetic resonance imaging
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DOI：
10.1016/j.mri.2016.10.030
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发表时间：
2017-02-01
影响因子：
2.5
通讯作者：
Murase, Kenya
中科院分区：
文献类型：
--
作者：
Saito, Shigeyoshi;Masuda, Kasumi;Murase, Kenya
背景：左室壁厚度是一项重要且常规测量的心脏学参数。在这里，我们介绍了使用自选通磁共振(MR)序列对小鼠心脏进行超高场MR电影成像的三维(3D)左室壁厚度和功能的标测。方法和结果：对6只雄性C57BL/6-j小鼠进行了11.7-T磁共振成像(MRI)。每只小鼠分别行短轴、长轴四腔、长轴双腔三个标准切面，从心尖到根部连续8次短轴扫描。在大约35分钟的观察期内，使用导航仪回波，将得到的11个自选通电影图像用于快速低角度拍摄分析。在短轴切面和四腔切面均可见右室和左室。在三维彩色标测图上测量室间隔壁(舒张期：0.94+/-0.05 mm，收缩期：1.20+/-0.09 mm)和左心室游离壁(舒张期：1.07+/-0.15 mm，收缩期：1.79+/-0.11 mm)厚度。基于超高场磁共振成像的自门控电影成像可以用来准确和容易地测量正常小鼠心脏的心功能和室壁厚度。在临床前研究中，这种通用和简单的方法将在临床上用于高场磁共振心功能和室壁厚度的评估。(C)2016 Elsevier Inc.保留所有权利。
Background: The left ventricle (LV) wall thickness is an important and routinely measured cardiologic parameter. Here we introduce three-dimensional (3D) mapping of LV wall thickness and function using a self-gated magnetic resonance (MR) sequence for ultra-high-field 11.7-T MR cine imaging of mouse hearts.Methods and results: Six male C57BL/6-j mice were subjected to 11.7-T MR imaging (MRI). Three standard views short axis, long axis four-chamber, and long axis two-chamber and eight consecutive short axis scans from the apex to base were performed for each mouse. The resulting 11 self-gated cine images were used for fast low-angle shot analysis with a navigator echo over an observation period of approximately 35 min. The right ventricle (RV) and LV were identified in the short axis and four-chamber views. On 3D color-coded maps, the interventricular septum wall (diastole: 0.94 +/- 0.05 mm, systole: 1.20 +/- 0.09 mm) and LV free wall (diastole: 1.07 +/- 0.15 mm, systole: 1.79 +/- 0.11 mm) thicknesses were measured.Conclusion: This 3D wall thickness mapping technique can be used to observe regional wall thickness at the end diastole and end-systole. Self-gated cine imaging based on ultra-high-field MRI can be used to accurately and easily measure cardiac function and wall thickness in normal mouse hearts. As in the preclinical study, this versatile and simple method will be clinically useful for the high-field-MRI evaluation of cardiac function and wall thickness. (C) 2016 Elsevier Inc. All rights reserved.