Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated children
Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated children
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DOI：
10.1099/13500872-145-8-2069
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发表时间：
1999-08-01
期刊：
影响因子：
--
通讯作者：
Mooi, FR
中科院分区：
文献类型：
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作者：
Mastrantonio, P;Spigaglia, P;Mooi, FR
百日咳杆菌在百日咳毒素(PT)S1亚基和百日咳毒素(PT)S1亚基这两种对免疫起重要作用的蛋白上表现出多态。先前的一项研究表明，在荷兰百日咳杆菌种群中，这些蛋白的抗原性发生了变化，并提出这些变化是由疫苗接种推动的。最近的意大利临床试验提供了机会来比较未接种疫苗的儿童和接种了两种无细胞百日咳疫苗和一种全细胞百日咳疫苗的儿童分离株中PRN和PT S1亚单位变异的频率。在所分析的129株病毒中发现了4个Prn变异体(Prn1、Prn2、Prn3和Prn5)。Prn1、Prn2和Prn3已经被描述过，而Prn5是一个新的变体。Prn1、Prn2、Prn3和Prn5分别在6、41、51和2%的菌株中检出。临床试验中用于生产疫苗的百日咳杆菌菌株被发现产生Prn1，即与Prn1在一个氨基酸上不同的一种类型。在疫苗接种组分离的菌株中，Prn1变异的频率最低，这表明Prn1菌株比PRn2和PRN3菌株更容易受到疫苗诱导免疫的影响。与疫苗株(S1B和S1D)产生的Pt51型不同，被检株中只有一株Pt51型(S1a)。在荷兰百日咳杆菌种群中，S1a型也占主导地位。以IS1002为基础的DNA指纹图谱分析百日咳杆菌菌株间的亲缘关系，发现有3种指纹型占优势，占菌株总数的70%以上。与PRN3相比，PRN2菌株表现出更多种类的指纹类型，这表明PRN3是最近出现的。根据疫苗驱动的进化，对结果进行了讨论。
Bordetella pertussis shows polymorphism in two proteins, pertactin (Prn) and the pertussis toxin (PT) S1 subunit, which are important for immunity. A previous study has shown antigenic shifts in these proteins in the Dutch B. pertussis population, and it was suggested that these shifts were driven by vaccination. The recent Italian clinical trial provided the opportunity to compare the frequencies of Prn and PT S1 subunit variants in strains isolated from unvaccinated children, and from children vaccinated with two acellular and one whole-cell pertussis vaccine. Four Prn variants (Prn1, Prn2, Prn3 and Prn5) were found in the 129 strains analysed. Prn1, Prn2 and Prn3 have been described previously, whereas Prn5 is a novel variant. Prn1, Prn2, Prn3 and Prn5 were found in, respectively, 6, 41, 51 and 2% of the strains. The B. pertussis strains used to produce the vaccines administered in the clinical trial were found to produce Prn1, or a type which differed from Prn1 in one amino acid. The frequency of the Prn1 variant was found to be lowest in the strains isolated from vaccinated groups, suggesting that Prn1 strains are more affected by vaccine-induced immunity than Prn2 and Prn3 strains. Only one PT 51 type (S1A) was observed in the examined strains, which was distinct from the types produced by the vaccine strains (S1B and S1D). The S1A type also predominates in the Dutch B. pertussis population. The genetic relationship among B. pertussis strains analysed by IS1002-based DNA fingerprinting revealed that three fingerprint types predominate, representing more than 70% of the strains. Prn2 strains showed a greater variety of fingerprint types compared to Prn3, suggesting that Prn3 has emerged more recently. The results are discussed in the light of vaccine-driven evolution.