Molecular analysis of the genetic relationship of trans interacting factors at the T/t complex.
Molecular analysis of the genetic relationship of trans interacting factors at the T/t complex.
复制标题
T/t 复合体反式相互作用因子遗传关系的分子分析。
作者：
Alton,AK;Silver,LM;Artzt,K;Bennett,D
T/ T复合体是小鼠17号染色体上靠近H-2复合体的一个广泛的遗传区域，对胚胎发育、精子发生和重组具有多重影响1 - 3。最近，睾丸细胞蛋白的二维凝胶分析在T/ T复合体中发现了一个基因，该基因编码一个主要的细胞表面相关蛋白p63/6.9（参考文献4）。野生型基因Tcp-1a编码63,000分子量的蛋白质（p63/6.9b），而突变等位基因Tcp-1a编码酸性更强的蛋白质（p63/6.9a），它存在于所有完整的倍型中。对罕见重组事件获得的部分倍型分析表明，attcp -1与尾部相互作用因子tt完全相关，该因子被认为是一个遗传等位基因ofT，因此提出了tof位点编码p63/6.9蛋白的可能性。我们在这里报道，p63/6.9蛋白由7条染色体产生，这些染色体在tof位点上携带独立衍生的显性突变，与野生型没有区别；因此，Ehe累积数据表明tcp -1基因很可能不在ofT位点。
The T/t complex is an extensive genetic region proximal to the H–2 complex on mouse chromosome 17, with multiple effects on embryonic development, spermatogenesis and recombination1–3. Recently, two-dimensional gel analysis of testicular cell proteins identified a gene within the T/t complex that codes for a major cell surface-associated protein, p63/6.9 (ref. 4). The wild-type gene,Tcp-1bcodes for a 63,000-motecular weight protein (p63/6.9b), whereas a mutant allele,Tcp-1a, which occurs in all intactthaplotypes, codes for a more acidic form of the protein (p63/6.9a). Analysis of partialthaplotypes obtained from rare recombination events showed thatTcp-1acorrelated completely with the tail interaction factortT, which is thought to be a genetic allele ofT, thus raising the possibility that the locus ofTcodes for the p63/6.9 protein. We report here that the p63/6.9 proteins produced by seven chromosomes carrying independently derived dominant mutations at the locus ofTare all indistinguishable from the wild-type form; thus, Ehe cumulative data indicate that theTcp-1gene is most probably not at the locus ofT.