Whole-central nervous system functional imaging in larval Drosophila.
Whole-central nervous system functional imaging in larval Drosophila.
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DOI：
10.1038/ncomms8924
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发表时间：
2015-08-11
影响因子：
16.6
通讯作者：
Keller PJ
中科院分区：
文献类型：
--
作者：
Lemon WC;Pulver SR;Höckendorf B;McDole K;Branson K;Freeman J;Keller PJ
理解大脑如何与中枢神经系统（CNS）的其他部分紧密协同工作，取决于对整个中枢神经系统协调活动模式的了解。我们提出了一种以高时空分辨率测量整个不透明中枢神经系统活动的方法。我们将一台能够以比现有技术快25倍的体成像速度进行同步多视角成像的光片显微镜、一种用于分离的果蝇幼虫中枢神经系统的全中枢神经系统成像检测方法以及一个用于分析多视角、全中枢神经系统钙成像数据的计算框架相结合。我们对大脑和腹神经索进行成像，分别以双光子或单光子激发以2Hz或5Hz的频率覆盖整个中枢神经系统。通过绘制虚拟行为期间的网络活动，并定量比较个体之间的高分辨率全中枢神经系统活动图谱，我们预测了中枢神经系统区域之间的功能连接，并揭示了大脑中能够识别腹神经索中执行的运动程序类型和时间状态的神经元。
要理解神经网络如何运作，在系统层面测量神经网络活动是很重要的。在这里，莱蒙等人开发了一个框架，该框架结合了高速多视角光片显微镜、一种全中枢神经系统成像检测方法和计算工具，以展示对整个分离的果蝇幼虫中枢神经系统的同步功能成像。
Understanding how the brain works in tight concert with the rest of the central nervous system (CNS) hinges upon knowledge of coordinated activity patterns across the whole CNS. We present a method for measuring activity in an entire, non-transparent CNS with high spatiotemporal resolution. We combine a light-sheet microscope capable of simultaneous multi-view imaging at volumetric speeds 25-fold faster than the state-of-the-art, a whole-CNS imaging assay for the isolated Drosophila larval CNS and a computational framework for analysing multi-view, whole-CNS calcium imaging data. We image both brain and ventral nerve cord, covering the entire CNS at 2 or 5 Hz with two- or one-photon excitation, respectively. By mapping network activity during fictive behaviours and quantitatively comparing high-resolution whole-CNS activity maps across individuals, we predict functional connections between CNS regions and reveal neurons in the brain that identify type and temporal state of motor programs executed in the ventral nerve cord. To understand how neuronal networks function, it is important to measure neuronal network activity at the systems level. Here Lemon et al. develop a framework that combines a high-speed multi-view light-sheet microscope, a whole-CNS imaging assay and computational tools to demonstrate simultaneous functional imaging across the entire isolated Drosophila larval CNS.