Linalool Inhibits LPS-Induced Inflammation in BV2 Microglia Cells by Activating Nrf2
Linalool Inhibits LPS-Induced Inflammation in BV2 Microglia Cells by Activating Nrf2
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DOI：
10.1007/s11064-015-1629-7
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发表时间：
2015-07-01
影响因子：
4.4
通讯作者：
Zheng, Yongri
中科院分区：
文献类型：
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作者：
Li, Yang;Lv, Ou;Zheng, Yongri
芳樟醇是精油的天然化合物，据报道具有抗炎作用。本研究旨在探讨芳樟醇对LPS刺激的BV 2小胶质细胞的抗炎作用及其机制。BV 2小胶质细胞在芳樟醇存在或不存在下用LPS刺激。检测炎症介质TNF-α、IL-1 β、NO和PGE（2）的产生以及Nrf 2、HO-1的表达。我们的研究结果表明芳樟醇以剂量依赖的方式抑制LPS诱导的TNF-α、IL-1 β、NO和PGE（2）的产生。芳樟醇还抑制LPS诱导的NF-κ B活化。芳樟醇处理诱导Nrf 2核转位和HO-1表达。此外，我们的研究结果表明，芳樟醇的抗炎作用减弱与Nrf 2 siRNA转染。总之，这些结果表明芳樟醇通过激活Nrf 2/HO-1信号通路抑制LPS诱导的BV 2小胶质细胞炎症。
Linalool, a natural compound of the essential oils, has been reported to have anti-inflammatory effects. This study aimed to investigate the anti-inflammatory effects and mechanism of linalool in LPS-stimulated BV2 microglia cells. BV2 microglia cells were stimulated with LPS in the presence or absence of linalool. The production of inflammatory mediators TNF-alpha, IL-1 beta, NO, and PGE(2) as well as Nrf2, HO-1 expression were detected. Our results showed that linalool inhibited LPS-induced TNF-alpha, IL-1 beta, NO, and PGE(2) production in a dose-dependent manner. Linalool also inhibited LPS-induced NF-kappa B activation. Treatment of linalool induced nuclear translocation of Nrf2 and expression of HO-1. In addition, our results showed that the anti-inflammatory effect of linalool was attenuated by transfection with Nrf2 siRNA. In conclusion, these results suggested that linalool inhibits LPS-induced inflammation in BV2 microglia cells by activating Nrf2/HO-1 signaling pathway.