HUMAN T-CELL LEUKEMIA-VIRUS MINUS STRAND TRANSCRIPTION IN INFECTED T-CELLS
HUMAN T-CELL LEUKEMIA-VIRUS MINUS STRAND TRANSCRIPTION IN INFECTED T-CELLS
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DOI：
10.1016/0006-291x(89)92322-x
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发表时间：
1989-09-15
影响因子：
3.1
通讯作者：
BRUNCK, TK
中科院分区：
文献类型：
--
作者：
LAROCCA, D;CHAO, LA;BRUNCK, TK
人类T细胞白血病病毒RNA基因组的正链编码对感染性、复制和转化至关重要的蛋白质。到目前为止，还没有发现HTLV-Ⅰ原病毒负链上编码的基因产物，我们已经鉴定了几个开放阅读框，即HTLV-Ⅰ负链上的序列。HTLV-I的3“末端显示在任一方向上促进测试基因表达。用与预测的转录区互补的单链RNA探测的RNA印迹显示，在HTLV-1感染的T细胞中有2.5和2.9个腺苷酸酶负链RNA，但在未感染的对照中没有。在通过突变分析研究人逆转录病毒蛋白功能时，也应考虑突变对负链基因产物的影响。
The plus strand of the Human T-cell Leukemia Virus RNA genome encodes proteins critical for infectivity, replication, and transformation. Thus far, gene products encoded on the minus strand of the HTLV-I provirus have not been found. We have identified several open reading frames, sequences on the minus strand of HTLV-I. The 3'' terminus of HTLV-I was shown to promote test gene expression in either orientation. RNA blots probed with single stranded RNA complementary to the predicted transcribed region revealed 2.5 and 2.9 kilobase minus strand RNAs in HTLV-I infected T-cells but not in an uninfected control. The effect of mutations on minus strand gene products should also be considered when studying human retroviral protein function by mutational analysis.