Fascin expression in human embryonic, fetal, and normal adult tissue
Fascin expression in human embryonic, fetal, and normal adult tissue
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DOI：
10.1369/jhc.7a7353.2007
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发表时间：
2008-02-01
影响因子：
3.2
通讯作者：
Li, En-Min
中科院分区：
文献类型：
--
作者：
Zhang, Fa-Ren;Tao, Li-Hua;Li, En-Min
本研究调查了肌成束蛋白在人类胚胎、胎儿和正常成人组织中的分布。采用组织微阵列技术对妊娠4-22周的人类胚胎和胎儿以及成人标本进行免疫组织化学实验。肌成束蛋白在神经系统中广泛表达。妊娠 4 周时，神经管中出现肌成束蛋白。妊娠 8-12 周时，小脑和胃肠道细胞中观察到同质基因表达。在发育后期和成人中，小脑和胃肠道腺上皮的浦肯野细胞没有表现出表达。妊娠8-12周时，肌成束蛋白在肾上腺的皮质和髓质中表达，而免疫反应性从肾小球带到网状带逐渐降低，并且在成人的肾上腺髓质中基本呈阴性。在神经元、淋巴组织的滤泡树突细胞、复层鳞状上皮的基底层细胞、间充质和血管内皮细胞的整个发育过程中观察到肌成束蛋白的显着表达。胆管、结肠、卵巢、胰腺和胃的单纯柱状上皮的肌成束蛋白表达均为阴性。这些结果表明肌成束蛋白的表达具有时间特异性和高度组织特异性。讨论了胚胎发生和癌发生中肌成束蛋白表达的相似性。
This study investigates the distribution of fascin inhuman embryonic, fetal, and normal adult tissues. Tissue microarray technology was used to perform immunohistochemical experiments on human embryos and fetuses at 4-22 weeks of gestation and adult specimens. Fascin was widely expressed in the nervous system. At 4 weeks of gestation, fascin was present in the neural tube. At 8-12 weeks of gestation, homogenous gene expression was seen in cells of the cerebellum and gastrointestinal tract. In later developmental stages and in adults, Purkinje cells of the cerebellum and glandular epithelium of the gastrointestinal tract showed no expression. Fascin was expressed in the cortex and medulla of the adrenal gland at 8-12 weeks of gestation, whereas immunoreactivity decreased from the zona glomerulosa through the zona reticularis and was essentially negative in the adrenal medulla of adults. Significant expression of fascin was seen throughout development in neurons, follicular dendritic cells of lymphoid tissue, basal layer cells of stratified squamous epithelia, mesenchyme, and vascular endothelial cells. Simple columnar epithelia of the biliary duct, colon, ovary, pancreas, and stomach were all negative for fascin expression. These results show that expression of fascin is time specific and highly tissue specific. Parallels between fascin expression in embryogenesis and carcinogenesis are discussed.