Simultaneous gut colonisation and infection by ESBL-producing Escherichia coli in hospitalised patients
Simultaneous gut colonisation and infection by ESBL-producing Escherichia coli in hospitalised patients
复制标题
DOI：
10.4066/amj.2015.2358
复制
发表时间：
2015-01-01
影响因子：
--
通讯作者：
Kanungo, Reba
中科院分区：
文献类型：
--
作者：
Asir, Johny;Nair, Shashikala;Kanungo, Reba
背景产超广谱β-内酰胺酶（ESBL）的细菌是医院感染的主要原因。产ESBL大肠埃希菌（E.大肠杆菌）的细菌。这些耐药微生物也被发现以细菌的形式存在于人类中。本研究旨在分离产ESBL的E.大肠埃希氏菌（E. coli）是大肠埃希氏菌的主要感染源，目前只有少数研究表明肠道菌群是大肠埃希氏菌感染的主要来源。大肠杆菌，是从肠道的病人谁已经被感染了同一organis.MethodsA共70个非重复粪便样本收集从我院的住院病人。这些住院患者均经临床诊断，产ESBL大肠埃希菌培养阳性。血液、尿液或脓液中的大肠杆菌。采用标准微生物学方法检测临床和肠道分离株中的ESBL。用聚合酶链反应（PCR）技术检测了产ESBL大肠杆菌的主要β内酰胺酶bla（CTX M）、bla（TEM）和bla（SHV）基因。从70个培养样本中的15个（21%）粪便样本中分离出大肠杆菌。PCR结果显示，在15株分离株中，有13株（86.6%）携带bla（CTX-M）基因，11株（73.3%）携带bla（TEM）基因，8株（53.3%）仅携带bla（SHV）基因。所有15个临床和肠道分离株具有相似的表型特征和15例患者中的8例具有相似的基因模式（bla（TEM），bla（CTX-M），和bla（SHV））在其临床和肠道分离株。
BackgroundExtended spectrum betalactamase (ESBL)-producing organisms are a major cause of hospital-acquired infections. ESBL-producing Escherichia coli (E. coli) have been recovered from the hospital environment. These drug-resistant organisms have also been found to be present in humans as commensals. The present investigation intended to isolate ESBL-producing E. coli from the gut of already infected patients; to date, only a few studies have shown evidence of the gut microflora as a major source of infection.AimsThis study aimed to detect the presence of ESBL genes in E. coli that are isolated from the gut of patients who have already been infected with the same organism.MethodsA total of 70 non-repetitive faecal samples were collected from in-patients of our hospital. These in-patients were clinically diagnosed and were culture-positive for ESBL-producing E. coli either from blood, urine, or pus. Standard microbiological methods were used to detect ESBL from clinical and gut isolates. Genes coding for major betalactamase enzymes such as bla(CTX-M), bla(TEM), and bla(SHV) were investigated by polymerase chain reaction (PCR).ResultsESBL-producing E. coli was isolated from 15 (21 per cent) faecal samples of the 70 samples that were cultured. PCR revealed that out of these 15 isolates, the bla(CTX-M) gene was found in 13 (86.6 per cent) isolates, the bla(TEM) was present in 11 (73.3 per cent) isolates, and bla(SHV) only in eight (53.3 per cent) isolates. All 15 clinical and gut isolates had similar phenotypic characters and eight of the 15 patients had similar pattern of genes (bla(TEM), bla(CTX-M), and bla(SHV)) in their clinical and gut isolates.ConclusionStrains with multiple betalactamase genes that colonise the gut of hospitalised patients are a potential threat and it may be a potential source of infection.