Modulation of proline-rich protein biosynthesis in rat parotid glands by sorghums with high tannin levels.
Modulation of proline-rich protein biosynthesis in rat parotid glands by sorghums with high tannin levels.
复制标题
高单宁水平的高粱对大鼠腮腺中富含脯氨酸的蛋白质生物合成的调节。
DOI：
10.1073/pnas.80.13.3948
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Carlson,DM
中科院分区：
文献类型：
--
作者：
Mehansho,H;Hagerman,A;Clements,S;Butler,L;Rogler,J;Carlson,DM
喂饲高单宁高粱（高单宁高粱）大鼠腮腺基因表达的变化类似于异丙肾上腺素治疗。在3天内，腮腺增大约3倍，一系列富含脯氨酸的蛋白质增加约12倍。与异丙肾上腺素治疗不同，未观察到颌下腺的变化，腮腺中的Mr 220，000糖蛋白未诱导。氨基酸分析，电泳图谱，和无细胞翻译的mRNA都证实，富含脯氨酸的蛋白质诱导喂养高单宁高粱是相同的异丙肾上腺素治疗引起的。富含脯氨酸的蛋白质的结合曲线单宁表现出亲和力10倍以上的牛血清白蛋白和单宁。
Feeding of sorghum with a high level of tannin (high-tannin sorghum) to rats caused changes in gene expression in parotid glands similar to isoproterenol treatment. Within 3 days the parotid glands were enlarged about 3-fold and a series of proline-rich proteins were increased about 12-fold. Unlike isoproterenol treatment, no changes were observed in the submandibular glands, and a Mr 220,000 glycoprotein in parotid glands was not induced. Amino acid analyses, electrophoretic patterns, and cell-free translations of mRNAs all confirmed that the proline-rich proteins induced by feeding high-tannin sorghum were identical to those induced by isoproterenol treatment. Binding curves for proline-rich proteins to tannins showed affinities 10-fold greater than bovine serum albumin and tannins.