Structure of the mouse gene encoding CD4 and an unusual transcript in brain.
Structure of the mouse gene encoding CD4 and an unusual transcript in brain.
复制标题
编码 CD4 的小鼠基因的结构和大脑中不寻常的转录物。
DOI：
10.1073/pnas.84.21.7644
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
Parnes,JR
中科院分区：
文献类型：
--
作者：
Gorman,SD;Tourvieille,B;Parnes,JR
T 细胞分化抗原 CD4 在识别 II 类主要组织相容性复合体蛋白的 T 细胞功能中发挥着重要作用。根据 cDNA 克隆预测的蛋白质序列，小鼠 CD4 (L3T4) 先前已被证明与免疫球蛋白可变区在进化上相关。人们发现编码 L3T4 的基因不仅在 T 谱系细胞的子集中转录，而且出人意料地也在大脑中转录，并在大脑中发现了较短的转录本。在本研究中，编码 L3T4 的基因跨度为 26 KB，包含 10 个外显子。除了在编码氨基末端免疫球蛋白样同源单位的序列中间显着存在内含子之外，其结构组织与免疫球蛋白基因超家族的其他成员相似。小鼠大脑中较短的 L3T4 转录物的结构已被确定。该 mRNA 似乎是从外显子 VI 编码序列内的转录起始位点产生的。如果翻译，该转录物将编码 217 个氨基酸的蛋白质，该蛋白质缺乏常见的 L3T4 信号肽和成熟蛋白质的氨基末端 214 个氨基酸。
The T-cell differentiation antigen CD4 plays an important role in the function of T cells that recognize class II major histocompatibility complex proteins. Mouse CD4 (L3T4) has previously been shown to be evolutionarily related to immunoglobulin variable regions based on the predicted protein sequence from cDNA clones. The gene encoding L3T4 was found to be transcribed not only in a subset of T-lineage cells but also unexpectedly in brain, where a shorter transcript was found. In the present study the gene encoding L3T4 is shown to span 26 kilobases and to contain 10 exons. The structural organization is similar to that of other members of the immunoglobulin gene superfamily except for the striking presence of an intron in the middle of the sequence encoding the amino-terminal immunoglobulin-like homology unit. The structure of the shorter L3T4 transcript in mouse brain has been determined. This mRNA appears to be generated from a transcriptional start site within the coding sequence in exon VI. If translated, this transcript would encode a protein of 217 amino acids that lacks the usual L3T4 signal peptide and the amino-terminal 214 amino acids of the mature protein.