Intraductal carcinoma is the precursor of carcinoma ex pleomorphic adenoma and is often associated with dysfunctional p53
Intraductal carcinoma is the precursor of carcinoma ex pleomorphic adenoma and is often associated with dysfunctional p53
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DOI：
10.1111/j.1365-2559.2007.02736.x
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发表时间：
2007-09-01
期刊：
影响因子：
6.4
通讯作者：
Harrison, J. D.
中科院分区：
文献类型：
--
作者：
Ihrler, S.;Weiler, C.;Harrison, J. D.
目的：虽然导管内癌已被证明在囊内癌不包括多形性腺瘤（CEPA），多形性腺瘤（PA）的转化CEPA的形态和遗传阶段不完全了解。本研究的目的是探讨囊内CEPA的形态学。方法和结果：囊内CEPA研究的最大系列进行免疫组化双重染色，检测p53蛋白和细胞增殖在不同类型的细胞结合突变分析的p53基因在激光显微切割材料。15/19例导管内癌伴高级别细胞浸润和p53蛋白频繁聚集。乳腺导管内癌8例。p53基因突变7/19例，其中5/15例为导管内癌。结论：导管内癌的发生率高，提示这种癌前病变可能是PA向CEPA恶变的一个恒定特征。这似乎是CEPA的一个特点，它是从初级和经常性PA发展而来的。结合免疫组化和遗传学数据显示，14/19例CEPA和11/15例导管内癌显示功能失调的p53基因或形态学证据，表明这是恶性转化的早期事件。
Aims: Although intraductal carcinoma has been demonstrated in intracapsular carcinoma ex pleomorphic adenoma (CEPA), the morphological and genetic stages of transformation of pleomorphic adenoma (PA) to CEPA are not fully understood. The aim of this study was to investigate the morphology of intracapsular CEPA.Methods and results: The largest series of intracapsular CEPA studied was subject to immunohistochemical double-staining to detect p53 protein and cellular proliferation in different types of cell combined with mutational analysis of the p53 gene in laser-microdissected material. Intraductal carcinoma with high-grade cellular atypia and frequent accumulation of p53 protein was found in 15/19 cases. Purely intraductal carcinoma was found in eight cases. Mutation of p53 was found in 7/19 cases, of which it was found in intraductal carcinoma in 5/15 cases.Conclusions: The frequent demonstration of intraductal carcinoma indicates that this preinvasive lesion is likely to be a constant feature in the malignant transformation of PA to CEPA. It appears to be a feature of CEPA developing from both primary and recurrent PA. The combined immunohistochemical and genetic data show that 14/19 cases of CEPA and 11/15 cases with intraductal carcinoma showed genetic or morphological evidence of dysfunctional p53, indicating that this is an early event in malignant transformation.