Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome
Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome
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DOI：
10.1186/s13071-018-2780-1
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发表时间：
2018-03-27
影响因子：
3.2
通讯作者：
Lambrechts, Louis
中科院分区：
文献类型：
--
作者：
Dickson, Laura B.;Ghozlane, Amine;Lambrechts, Louis
背景：宿主相关微生物，统称微生物群，在多细胞生物生物学中起着重要作用。在人类病原体的蚊子媒介中，肠道细菌微生物群影响媒介能力，已成为研究的热点。在媒介生物学的实验室研究中，遗传效应通常是根据在同一昆虫箱内饲养的蚊子的地理和遗传多样性群体之间的差异来推断的。然而，目前尚不清楚的是，蚊子菌落中微生物群组成不受控制的差异会在多大程度上混淆遗传效应。为了解决这个问题，我们使用16S元基因组学比较了最近从野生种群中获得的六个埃及伊蚊实验室菌落的中肠细菌微生物群，这些菌落代表了该物种的地理范围和遗传多样性。结果：我们发现伊蚊6个不同菌落的中肠细菌微生物群的多样性、丰度和群落结构非常相似。埃及伊蚊，不论其地理来源如何。我们还证实了居住在蚊子中肠的细菌群落相对较低的复杂性。结论：我们的研究结果表明，伊蚊的种群分布具有地理多样性。在同一虫港饲养的埃及伊蚊具有相似的肠道细菌微生物群，这支持了成年蚊子的肠道微生物群是由环境决定的这一结论，而与宿主基因型无关。因此，微生物群组成中不受控制的差异不太可能在媒介生物学的遗传研究中代表一个重要的混淆因素。
Background: Host-associated microbes, collectively known as the microbiota, play an important role in the biology of multicellular organisms. In mosquito vectors of human pathogens, the gut bacterial microbiota influences vectorial capacity and has become the subject of intense study. In laboratory studies of vector biology, genetic effects are often inferred from differences between geographically and genetically diverse colonies of mosquitoes that are reared in the same insectary. It is unclear, however, to what extent genetic effects can be confounded by uncontrolled differences in the microbiota composition among mosquito colonies. To address this question, we used 16S metagenomics to compare the midgut bacterial microbiome of six laboratory colonies of Aedes aegypti recently derived from wild populations representing the geographical range and genetic diversity of the species.Results: We found that the diversity, abundance, and community structure of the midgut bacterial microbiome was remarkably similar among the six different colonies of Ae. aegypti, regardless of their geographical origin. We also confirmed the relatively low complexity of bacterial communities inhabiting the mosquito midgut.Conclusions: Our finding that geographically diverse colonies of Ae. aegypti reared in the same insectary harbor a similar gut bacterial microbiome supports the conclusion that the gut microbiota of adult mosquitoes is environmentally determined regardless of the host genotype. Thus, uncontrolled differences in microbiota composition are unlikely to represent a significant confounding factor in genetic studies of vector biology.