Spatial patterns of olfactory bulb single-unit responses to learned olfactory cues in young rats.
Spatial patterns of olfactory bulb single-unit responses to learned olfactory cues in young rats.
复制标题
幼鼠嗅球单个单元对习得嗅觉线索反应的空间模式。
DOI：
10.1152/jn.1988.59.6.1770
复制
发表时间：
1988
影响因子：
2.5
通讯作者：
Leon,M
中科院分区：
文献类型：
--
作者：
Wilson,DA;Leon,M
1. 从出生第1天（PN1）到PN18天，新生大鼠幼崽典型地适应气味刺激。非条件刺激采用触觉刺激（抚摸）。在PN19上，研究了条件和对照幼崽的二尖瓣/簇状细胞对条件气味的单单位反应。在嗅球的两个区域的二尖瓣/丛状细胞中进行记录：1)通常与对条件气味的局灶[14C]2-脱氧葡萄糖（2- dg）摄取相关的区域，2)远离局灶2- dg摄取到条件气味的区域。在单单元记录过程中，动物用聚氨酯麻醉，自然呼吸。2. 二尖瓣/簇状细胞对气味的反应率的变化在球区和所有训练组被分类为兴奋性、抑制性或无反应。这种反应分类被用来比较球区和训练组对条件气味的反应模式。3. 经典条件反射选择性地改变了二尖瓣/簇状细胞对条件气味的反应模式，当这些细胞与对该气味的局灶2-DG摄取区域相关时。与对照组相比，二尖瓣/簇状细胞对条件气味的抑制反应明显增加，兴奋反应明显减少。对新气味的反应模式没有类似的改变。4. 与对照组相比，远离2-DG摄取焦点区域的二尖瓣/簇状细胞对条件气味的反应模式没有被条件改变。5. 在刺激开始后500 ms内，2-DG病灶内的细胞和远离2-DG病灶的细胞之间的反应模式差异是明显的。考虑到这些幼崽的呼吸频率（2hz），这些数据表明，在第一次吸入习得的气味时发生了改变的反应模式。6. 这些数据表明，嗅球输出神经元活动的空间和时间模式都被用于嗅球中嗅觉信息的编码。此外，这些空间/时间反应模式可以通过早期学习来改变。
1. Neonatal rat pups were classically conditioned to an odor stimulus from postnatal day 1 (PN1) to PN18. Tactile stimulation (stroking) was used as the unconditioned stimulus. On PN19, mitral/tufted cell single-unit responses to the conditioned odor were examined in both conditioned and control pups. Recordings were made from mitral/tufted cells in two regions of the olfactory bulb: 1) an area typically associated with focal [14C]2-deoxyglucose (2-DG) uptake in response to the conditioned odor and 2) an area distant from focal 2-DG uptake to the conditioned odor. Animals were anesthetized with urethane and were naturally respiring during the single-unit recording procedure. 2. Changes in mitral/tufted cell firing rate in response to odors in both bulbar regions and all training groups were classified as either excitatory, suppressive, or no response. This response classification was used to compare response patterns to the conditioned odor between bulbar regions and training groups. 3. Classical conditioning selectively modified the response patterns of mitral/tufted cells to the conditioned odor when those cells were associated with regions of focal 2-DG uptake for that odor. Mitral/tufted cells demonstrated significantly more suppressive and fewer excitatory responses to the conditioned odor than cells in control pups. Response patterns to a novel odor were not similarly modified. 4. Response patterns of mitral/tufted cells distant from the focal region of 2-DG uptake to the conditioned odor were not modified by conditioning compared with control pups. 5. The difference in response pattern between cells in the 2-DG focus and cells distant to the 2-DG focus was apparent within 500 ms of the stimulus onset. Given the respiratory rate of these pups (2 Hz), these data suggest that the modified response pattern occurred on the first inhalation of the learned odor. 6. These data demonstrate that both spatial and temporal patterns of olfactory bulb output neuron activity are used in the coding of olfactory information in the bulb. Furthermore, these spatial/temporal response patterns can be modified by early learning.