How molecular motors work in the crowded environment of living cells: coexistence and efficiency of normal and anomalous transport.
How molecular motors work in the crowded environment of living cells: coexistence and efficiency of normal and anomalous transport.
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DOI：
10.1371/journal.pone.0091700
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Metzler R
中科院分区：
文献类型：
--
作者：
Goychuk I;Kharchenko VO;Metzler R
最近的实验揭示了各种纳米颗粒的被动亚扩散和分子马达在活生物细胞的分子拥挤环境中对这些颗粒的异常主动运输。被动和主动微观流变学揭示，这种异常动力学的起源是由于细胞内流体的粘弹性。分子马达在这样一个高粘性、耗散性的环境中是如何工作的呢？我们能否解释所观察到的100至500 nm大小的相对较大的颗粒的驱动蛋白马达的异常运输与相同的分子马达的较小颗粒的正常运输的共存？在反常输运状态下分子马达的效率是多少？在这里，我们回答这些看似矛盾的问题，并始终解释实验结果的推广，著名的连续扩散模型的分子马达与两个构象状态，其中包括粘弹性效应。
Recent experiments reveal both passive subdiffusion of various nanoparticles and anomalous active transport of such particles by molecular motors in the molecularly crowded environment of living biological cells. Passive and active microrheology reveals that the origin of this anomalous dynamics is due to the viscoelasticity of the intracellular fluid. How do molecular motors perform in such a highly viscous, dissipative environment? Can we explain the observed co-existence of the anomalous transport of relatively large particles of 100 to 500 nm in size by kinesin motors with the normal transport of smaller particles by the same molecular motors? What is the efficiency of molecular motors in the anomalous transport regime? Here we answer these seemingly conflicting questions and consistently explain experimental findings in a generalization of the well-known continuous diffusion model for molecular motors with two conformational states in which viscoelastic effects are included.
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影响因子：
3.3
作者：
Jeon, Jae-Hyung;Leijnse, Natascha;Metzler, Ralf
通讯作者：
Metzler, Ralf
影响因子：
8.6
作者：
Amblard, F;Maggs, AC;Leibler, S
通讯作者：
Leibler, S
影响因子：
18.1
作者：
KUBO, R
通讯作者：
KUBO, R
影响因子：
3.5
作者：
Barkai, Eli;Garini, Yuval;Metzler, Ralf
通讯作者：
Metzler, Ralf
DOI：
10.1073/pnas.141080498
发表时间：
2001-07-03
影响因子：
11.1
作者：
Fisher, ME;Kolomeisky, AB
通讯作者：
Kolomeisky, AB