Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina
Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina
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DOI：
10.1083/jcb.201604095
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发表时间：
2016-10-24
影响因子：
7.8
通讯作者：
Norden, Caren
中科院分区：
文献类型：
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作者：
Icha, Jaroslav;Kunath, Christiane;Norden, Caren
将神经元排列成不同的层对于神经元的连接和功能至关重要。在发育过程中，大多数神经元从它们的出生地移动到适当的层，在那里它们生长。然而，许多神经元易位事件的动力学和模式仍有待探索。在这项研究中，我们研究视网膜神经节细胞（RGC）跨胚胎斑马鱼视网膜的易位。在完成它们的移位后，RGC建立最基底的视网膜层，在那里它们形成视神经。使用全光片显微镜，我们表明，RGCs的染色体易位是一个快速和定向的事件。它依赖于基底突起附着和稳定的微管。有趣的是，干扰染色体易位诱导转换为多极迁移。这种多极模式效率较低，但仍然导致成功的RGC层形成。当这两种模式都被抑制时，RGC不能移位并诱导层压缺陷。这表明正确的RGC易位对随后的视网膜分层至关重要。
The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) trans location across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RGCs is a fast and directed event. It depends on basal process attachment and stabilized microtubules. Interestingly, interference with somal translocation induces a switch to multipolar migration. This multipolar mode is less efficient but still leads to successful RGC layer formation. When both modes are inhibited though, RGCs fail to translocate and induce lamination defects. This indicates that correct RGC translocation is crucial for subsequent retinal lamination.