Comparison of dosage-dependent effects of beta-aminopropionitrile, sodium fluoride, and hydrocortisone on selected physical properties of cortical bone.
Comparison of dosage-dependent effects of beta-aminopropionitrile, sodium fluoride, and hydrocortisone on selected physical properties of cortical bone.
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β-氨基丙腈、氟化钠和氢化可的松对皮质骨选定物理性质的剂量依赖性影响的比较。
DOI：
10.1002/jbmr.5650090909
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Mook,HA
中科院分区：
文献类型：
--
作者：
Lees,S;Hanson,D;Page,E;Mook,HA
据报道，BAPN、氟化钠和氢化可的松可诱导矿化状态改变。使用雄性新西兰白色兔进行三组单独的实验，每种试剂一组。在每项实验中，将家兔分组，每只家兔以规定的体重测定剂量饲喂13周，然后处死。对左侧股骨和胫骨的密质骨进行密度、成分、声速、纵向弹性模量、矿化胶原的赤道衍射间距、骨干横截面积和相对载荷应力测试。β-氨基丙腈（BAPN）在所有剂量水平下均诱导所有性质的单调降解，对应于密度随剂量水平降低。胶原的弹性模量降低，赤道衍射间距增加。横截面骨干类似于编织骨。性质的变异性随着剂量的增加而增加。给定重量的总横截面积增加，这意味着弹性性能的降低被更大的面积所补偿，以支撑重量。氟处理家兔的平均密度和其他特性略有增加，在20 mg/kg BW/天时达到峰值。对于更高的剂量，性能降低，并且在高氟化物剂量下的值比BAPN低得多。赤道衍射间距无峰，随剂量增加而增大。据推测，氟磷灰石比普通磷灰石矿物更致密，因此具有更小的比容。氟化矿物的重量分数越大，其体积分数就越小。骨切片看起来更正常，除了多孔。当骨密度增加时，总横截面积减小，然后随着密度福尔斯降低而增加，这再次表明支撑体重所需的面积取决于弹性模量的大小。氢化可的松处理家兔的某些骨特性发生了微小变化，但在程序的不确定性范围内，对其他骨特性的影响无法检测到。骨干横截面积无变化。
BAPN, sodium fluoride, and hydrocortisone are reported to induce altered mineralization states. Three separate sets of experiments, one set for each agent, were performed using male New Zealand white rabbits. In each experiment the rabbits were segregated into groups, each fed a specified weight‐determined dose for 13 weeks and then sacrificed. Compact bone from the left femur and tibia were tested for density, composition, sonic velocity, longitudinal elastic modulus, equatorial diffraction spacing of mineralized collagen, diaphyseal cross‐sectional area, and relative load stress. β‐Aminopropionitrile (BAPN) induced monotonic degradation of all properties at all dose levels, corresponding to the decreasing density with dosage level. The elastic moduli show a decrease; the equatorial diffraction spacing of the collagen increases. The cross‐sectioned diaphysis resembled woven bone. The variability in properties increased with dosage. The total cross‐sectional area for a given weight increased, implying that the decreased elastic properties were compensated for by a larger area to support the weight. There was a slight increase in average density and other properties for fluoride‐treated rabbits, peaking at 20 mg/kg BW/day. For higher dosages the properties are degraded and the values were much lower at high fluoride dosages than for BAPN. There was no peak for the equatorial diffraction spacing, which increased with dosage. It is inferred that the fluorosed apatite is denser than normal apatitic mineral and therefore has a smaller specific volume. A greater weight fraction of fluorosed mineral has a smaller volume fraction than the equivalent normal apatitic mineral. The bone sections look more normal, except for the porosis. The total cross‐sectional area decreases when the bone density increases and then increases as the density falls, again implying that the area required to support body weight depends on the magnitude of the elastic moduli. There was a small change in some of the properties of the bones of the hydrocortisone‐treated rabbits, but the effects on others were undetectable within the uncertainty of the procedures. There was no change in the cross‐sectional areas of the diaphyses.