The Regulatory Network of CMPG1-V in Wheat-Blumeria graminis f. sp. tritici Interaction Revealed by Temporal Profiling Using RNA-Seq.
The Regulatory Network of CMPG1-V in Wheat-Blumeria graminis f. sp. tritici Interaction Revealed by Temporal Profiling Using RNA-Seq.
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DOI：
10.3390/ijms21175967
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发表时间：
2020-08-19
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Liu J;Sun L;Chen Y;Wei L;Hao Y;Yu Z;Wang Z;Zhang H;Zhang X;Li M;Wang H;Xiao J;Wang X
小麦白粉病（Pm）是一种常见的真菌病，由小麦蓝孢杆菌（Blumeria graminis f. sp. tritici, Bgt）引起。小麦的二倍体亲缘种Haynaldia villosa （H. villosa）对Pm具有广谱抗性。先前的研究报道了来自H. villosa的E3连接酶基因CMPG1-V，显示出对Pm的BSR。为了阐明CMPG1-V介导的调控网络，本研究分析比较了接种Bgt后4个侵染阶段CMPG1-V转基因植株（CMPG1-VOE）及其受体扬麦158的基因表达谱。GO和KEGG分析显示，与扬麦158相比，CMPG1-VOE在SA和ABA信号、淀粉/蔗糖代谢和光合作用方面存在明显的重编程。SA合成基因SARD1和UGT、信号因子TGA和PRs以及ABA信号通路中的SnRKs的转录本在CMPG1-VOE而不是Yangmai 158中特异性上调。光合作用中的LHCII转录、淀粉/蔗糖代谢中的GLUC转录和TPP转录在CMPG1-VOE中也有明显的诱导。WGCNA分析显示了CMPG1-V的关键调控候选基因，包括丝氨酸/苏氨酸蛋白激酶磷酸化、类黄酮生物合成中的葡萄糖基转移酶、防御因子WRKYs和氧化应激中的过氧化物酶。我们的研究结果有助于破译CMPG1-V的抗性调控网络，并确定可能用于育种计划的关键候选基因。
Wheat powdery mildew (Pm), caused by Blumeria graminis f. sp. tritici (Bgt), is a prevalent fungal disease. The diploid wheat relative Haynaldia villosa (H. villosa) showed broad-spectrum resistance (BSR) to Pm. A previous study reported an E3 ligase gene, CMPG1-V from H. villosa, showing BSR to Pm. To elucidate the regulatory network mediated by CMPG1-V, in this study, gene expression profiling of CMPG1-V transgenic plant (CMPG1-VOE) and its receptor Yangmai 158 was analyzed and compared after Bgt inoculation at four infection stages. GO and KEGG analysis revealed obvious reprogramming of SA and ABA signaling, starch/sucrose metabolism, and photosynthesis in CMPG1-VOE, compared with those in Yangmai 158. Transcripts of SA synthesis genes SARD1 and UGT, signaling factors TGA and PRs, and SnRKs in ABA signaling were specifically upregulated in CMPG1-VOE rather than Yangmai 158. Transcripts of LHCII in photosynthesis, GLUC and TPP in starch/sucrose metabolism were also induced distinctly in CMPG1-VOE. WGCNA analysis showed crucial regulatory candidates of CMPG1-V, involving serine/threonine-protein kinase in phosphorylation, glucosyltransferase in flavonoid biosynthesis, defense factor WRKYs, and peroxidase in oxidative stress. Our results facilitate the deciphering of the resistant regulatory network of CMPG1-V and the identification of key candidates which might be employed in breeding programs.
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