Microtubule organization is determined by the shape of epithelial cells.
Microtubule organization is determined by the shape of epithelial cells.
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DOI：
10.1038/ncomms13172
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发表时间：
2016-10-25
影响因子：
16.6
通讯作者：
Brown NH
中科院分区：
文献类型：
--
作者：
Gomez JM;Chumakova L;Bulgakova NA;Brown NH
间期微管组织对于细胞功能和组织结构至关重要。一般来说，物理机制足以驱动单个细胞中的微管组织，而组织中的细胞被认为利用信号传导机制。通过改善发育中的果蝇胚胎内微管排列的成像和定量，在这里，我们证明了上皮细胞顶面下的微管排列遵循细胞形状。在发育过程中，表皮细胞伸长和微管排列同时发生，但通过扰动细胞形状，我们发现微管组织响应细胞形状，而不是匡威。一组简单的微管行为规则足以使计算机模型模拟观察到的细胞表面几何形状变化的反应。此外，我们表明，微管与细胞边界碰撞拉链或depressed在一个角度依赖性的方式，如模型预测。最后，我们表明微管排列响应于不同上皮细胞的形状。 间期微管组织产生了一个结构支架，用于确定细胞极性和细胞形状的货物递送。在这里，作者表明，在果蝇上皮细胞的微管的方向响应于细胞的形状，而不是匡威，并开发了一个简单的模型来模拟这种行为。
Interphase microtubule organization is critical for cell function and tissue architecture. In general, physical mechanisms are sufficient to drive microtubule organization in single cells, whereas cells within tissues are thought to utilize signalling mechanisms. By improving the imaging and quantitation of microtubule alignment within developing Drosophila embryos, here we demonstrate that microtubule alignment underneath the apical surface of epithelial cells follows cell shape. During development, epidermal cell elongation and microtubule alignment occur simultaneously, but by perturbing cell shape, we discover that microtubule organization responds to cell shape, rather than the converse. A simple set of microtubule behaviour rules is sufficient for a computer model to mimic the observed responses to changes in cell surface geometry. Moreover, we show that microtubules colliding with cell boundaries zip-up or depolymerize in an angle-dependent manner, as predicted by the model. Finally, we show microtubule alignment responds to cell shape in diverse epithelia. Interphase microtubule organization creates a structural scaffold for cargo delivery determining cell polarity and cell shape. Here the authors show that in Drosophila epithelia the orientation of microtubules responds to cell shape, rather than the converse, and develop a simple model to mimic this behaviour.