Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis
Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis
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DOI：
10.1016/j.virusres.2008.12.008
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发表时间：
2009-04-01
期刊：
影响因子：
5
通讯作者：
Chauhan, R. S.
中科院分区：
文献类型：
--
作者：
Ravindra, P. V.;Tiwari, Ashok K.;Chauhan, R. S.
强毒株纽卡斯尔病病毒（NDV）在各种鸟类中引起高度传染性和经济上显著的纽卡斯尔病（ND）。在细胞培养中，NDV诱导细胞病变效应（CPE），其特征在于变圆、空泡化、合胞体形成和细胞死亡。除了由病毒的F和HN糖蛋白引起的细胞与细胞融合外，导致细胞死亡的分子事件尚不清楚。在目前的研究中，NDV感染的Vero细胞，在感染后48小时，细胞核浓缩，胞浆起泡，DNA断裂，磷脂酰丝氨酸易位到细胞表面。此外，病毒感染的细胞表现出减少的DNA含量和增加的Bax Bcl-2的比例，p53水平和caspase 3，8，9的表达相比，模拟感染的细胞。基于这些结果，可以得出结论，NDV感染细胞中的CPE是由p53和Bax依赖的凋亡途径参与的凋亡诱导引起的。(C)2008 Elsevier B. V.保留所有权利。
The velogenic Newcastle disease virus (NDV) causes highly infectious and economically significant Newcastle disease (ND) in birds of various species. In cell culture NDV induces cytopathic effect (CPE) characterized by rounding, vacuolation, syncytia formation and cell death. Aside from cell to cell fusion caused by the F and HN glycoprotein of the virus molecular events leading to cell death are not known. In the current study, NDV-infected Vero cells, at 48 h p.i., showed nuclear condensation, cytoplasm blebbing, DNA fragmentation, and phosphatidylserine translocation to the cell surface. In addition, virus-infected cells demonstrated decreased DNA content and an increased Bax to Bcl-2 ratio, p53 level and caspase 3, 8, 9 expression compared to mock-infected cells. Based on these results, it was concluded that CPE in NDV-infected cells was caused by to the induction of apoptosis with the involvement of p53 and the Bax, dependent apoptotic pathways. (C) 2008 Elsevier B.V. All rights reserved.