Cbx3 maintains lineage specificity during neural differentiation.
Cbx3 maintains lineage specificity during neural differentiation.
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Cbx3 在神经分化过程中保持谱系特异性
DOI：
10.1101/gad.292169.116
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发表时间：
2017-02-01
影响因子：
10.5
通讯作者：
Carey M
中科院分区：
文献类型：
--
作者：
Huang C;Su T;Xue Y;Cheng C;Lay FD;McKee RA;Li M;Vashisht A;Wohlschlegel J;Novitch BG;Plath K;Kurdistani SK;Carey M
在这项研究中，Huang等人研究了染色体盒同源物3（Cbx 3）刺激细胞分化的机制。他们发现，在小鼠ESC分化为神经祖细胞后，Cbx 3与基因启动子结合，并招募了介体亚基Med 26，从而证明Cbx 3和Med 26共同确保了谱系特化的保真度。染色体盒同源物3（Cbx 3/异染色质蛋白1γ [HP 1 γ]）刺激细胞分化，但其机制尚不清楚。我们发现Cbx 3在小鼠胚胎干细胞（ESC）分化为神经祖细胞（NPC）后与基因启动子结合，并招募中介子亚基Med 26。RNAi敲低Cbx 3或Med 26抑制神经分化，同时上调参与中胚层谱系决定的基因。因此，Cbx 3和Med 26一起通过增强神经基因的表达和下调对替代命运特异性的基因来确保谱系特异性的保真度。
In this study, Huang et al. investigated the mechanisms by which chromobox homolog 3 (Cbx3) stimulates cell differentiation. They found that Cbx3 binds to gene promoters upon differentiation of murine ESCs to neural progenitors and recruits the Mediator subunit Med26, thus demonstrating that Cbx3 and Med26 together ensure the fidelity of lineage specification. Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26. RNAi knockdown of either Cbx3 or Med26 inhibits neural differentiation while up-regulating genes involved in mesodermal lineage decisions. Thus, Cbx3 and Med26 together ensure the fidelity of lineage specification by enhancing the expression of neural genes and down-regulating genes specific to alternative fates.