Rejuvenation of chondrogenic potential in a young stem cell microenvironment
Rejuvenation of chondrogenic potential in a young stem cell microenvironment
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DOI：
10.1016/j.biomaterials.2013.09.099
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发表时间：
2014-01-01
期刊：
影响因子：
14
通讯作者：
Pei, Ming
中科院分区：
文献类型：
--
作者：
Li, Jingting;Hansen, Kirk C.;Pei, Ming
自体细胞在用于软骨修复的离体扩增过程中会受到细胞数量有限和衰老的影响。在这里，我们发现胎儿滑膜衍生干细胞（SDSC）（FE）沉积的细胞外基质（ECM）的扩张在促进细胞增殖和软骨形成潜力方面优于成人SDSC（AE）沉积的ECM。 FE 中的独特蛋白质可能是 FE 的返老还童效果的原因，而 AE 中的有利蛋白质可能对分化的贡献大于对增殖的贡献。与AE相比，FE的较低弹性产生了具有较低弹性的扩张的成年SDSC，这可能是软骨形成和脂肪形成分化增强的原因。 MAPK 和非经典 Wnt 信号积极参与 ECM 介导的成人 SDSC 返老还童。 (C) 2013 Elsevier Ltd. 保留所有权利。
Autologous cells suffer from limited cell number and senescence during ex vivo expansion for cartilage repair. Here we found that expansion on extracellular matrix (ECM) deposited by fetal synovium-derived stem cells (SDSCs) (FE) was superior to ECM deposited by adult SDSCs (AE) in promoting cell proliferation and chondrogenic potential. Unique proteins in FE might be responsible for the rejuvenation effect of FE while advantageous proteins in AE might contribute to differentiation more than to proliferation. Compared to AE, the lower elasticity of FE yielded expanded adult SDSCs with lower elasticity which could be responsible for the enhancement of chondrogenic and adipogenic differentiation. MAPK and noncanonical Wnt signals were actively involved in ECM-mediated adult SDSC rejuvenation. (C) 2013 Elsevier Ltd. All rights reserved.