Biochemical and functional characterization of transiently expressed in neural precursor (TENP) protein in emu egg white.
Biochemical and functional characterization of transiently expressed in neural precursor (TENP) protein in emu egg white.
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DOI：
10.1021/jf5008117
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发表时间：
2014-05
影响因子：
6.1
通讯作者：
K. Maehashi;Megumi Ueda;M. Matano;J. Takeuchi;M. Uchino;Y. Kashiwagi;Toshihiro Watanabe
中科院分区：
文献类型：
--
作者：
K. Maehashi;Megumi Ueda;M. Matano;J. Takeuchi;M. Uchino;Y. Kashiwagi;Toshihiro Watanabe
一种在发育中组织的神经前体中瞬时表达的蛋白质(TENP)被发现作为主要蛋白质之一存在于鳗(Dromaius Novaehollandiae)的卵清中。核苷酸序列分析表明，该基因编码452个氨基酸，含有19个氨基酸的多肽信号。系统发育分析表明，emu TENP与其他禽类TENP一起属于杀菌/通透性增加蛋白(BPI)超家族。RT-PCR分析表明，EMU TENP基因在输卵管枕大部高度表达，表明TENP是蛋清的主要成分。通过阴离子交换层析和硫酸铵分级分离纯化了EMU TENP。与BPI不同，EMU TENP对革兰氏阳性菌(包括黄色微球菌和枯草杆菌)具有抗菌活性，但对革兰氏阴性菌(如大肠杆菌和鼠伤寒沙门氏菌)没有抗菌活性。结果表明，EMU TENP是一种新型的高效抗菌蛋白，具有与BPI不同的光谱特征。
A protein transiently expressed in the neural precursors of developing tissues (TENP) was found to be present in emu (Dromaius novaehollandiae) egg white as one of the major proteins. Nucleotide analysis of its encoding cDNA revealed a sequence of 452 amino acids including a 19 amino acid peptide signal. Phylogenetic analysis determined that emu TENP was clustered within the bactericidal/permeability-increasing protein (BPI) superfamily together with other avian TENPs. RT-PCR analysis revealed that the emu TENP gene was highly expressed in the magnum of the oviduct, indicating that TENP is a major egg white component. Emu TENP was purified by anion exchange chromatography and ammonium sulfate fractionation. Unlike BPI, emu TENP exhibited antibacterial activity against Gram-positive bacteria, including Micrococcus luteus and Bacillus subtilis, but not against Gram-negative bacteria such as Escherichia coli and Salmonella Typhimurium. The results suggest that emu TENP is a potent novel antibacterial protein with a spectrum distinct from that of BPI.