Production of phospholipase C (alpha-toxin), haemolysins and lethal toxins by Clostridium perfringens types A to D.
Production of phospholipase C (alpha-toxin), haemolysins and lethal toxins by Clostridium perfringens types A to D.
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A 型至 D 型产气荚膜梭菌产生磷脂酶 C（α 毒素）、溶血素和致死毒素。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
T. Wadström
中科院分区：
文献类型：
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作者：
R. Möllby;T. Holme;C. Nord;C. Smyth;T. Wadström
为了获得高产量的细胞外酶和毒素用于免疫分析，在控制条件下，在预还原的蛋白酶蛋白胨培养基中，在发酵罐中培养了产气荚膜梭菌A型至D型和28株新鲜的人产气荚膜梭菌A型菌株。类型培养收集菌株在生长速率和生长最适pH值方面均表现出不同的特征。磷脂酶C （α毒素）、溶血素的产生和致死活性在不同类型之间差别很大。比较了pH控制和静态或搅拌培养下发酵罐的生长和细胞外蛋白产量。在pH控制的发酵罐中培养，所有细胞外蛋白的产量都得到了显著提高。菌株ATCC13124产生的磷脂酶C比在相同条件下生长的28株新分离的A型产气荚膜荚膜杆菌中的任何一株多5倍。ATCC菌株的溶血和致死活性等于或优于任何新分离菌株的活性。从临床气性坏疽和腹部伤口病例中分离的A型菌株与从健康人粪便样本中分离的A型菌株在细菌产量和细胞外毒素的产生方面没有差异。
To obtain high yields of extracellular enzymes and toxins for immunological analysis, type culture collection strains of Clostridium perfringens types A to D and 28 fresh isolates of C. perfringens type A from humans were grown in fermenters under controlled conditions in a pre-reduced proteose peptone medium. The type culture collection strains all showed different characteristics with respect to growth rates and pH optima for growth. Production of phospholipase C (alpha-toxin), haemolysin and lethal activity varied considerably between the different types. Growth and extracellular protein production in fermenters with pH control and static or stirred cultures were compared. Production of all extracellular proteins measured was markedly improved by cultivation in fermenters with pH control. Strain ATCC13124 produced five times more phospholipase C than any of 28 freshly isolated strains of C. perfringens type A, grown under identical conditions. Haemolytic and lethal activities of the ATCC strain were equal or superior to the activities of any of the freshly isolated strains. There were no differences in the bacterial yields and in the production of extracellular toxins between type A strains isolated from clinical cases of gas gangrene and abdominal wounds, and those isolated from faecal samples from healthy persons.