Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.
Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.
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酿酒酵母 DIS3 突变体中存在明显且广泛的微管缺陷。
DOI：
10.1002/yea.1899
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Andrulis,ErikD
中科院分区：
文献类型：
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作者：
Smith,SarahB;Kiss,DanielL;Turk,Edward;Tartakoff,AlanM;Andrulis,ErikD
RNA加工外泌体的亚基组装成结构不同的蛋白质复合物，其在不同的细胞区室和RNA代谢途径中发挥作用。在这里，通过遗传学、细胞生物学和转录组学分析，我们研究了Dis 3在细胞周期进程中的作用，Dis 3是一种具有内切和3′ → 5′外切核糖核酸酶活性的必需多肽。我们提出了几条线的证据表明，扰动DIS 3影响微管（MT）的定位和结构inSaccharomycescerevisiae。具有DIS 3突变体的细胞：（a）积累后期和后期前有丝分裂纺锤体;（B）表现出纺锤体错误定向并从芽颈移位;（c）具有伸长的纺锤体相关星形MT;（d）具有增加的G1星形MT长度和数量;以及（e）对MT毒物过敏。核心外泌体基因RRP 4和MTR 3以及外泌体辅因子基因MTR 4中的突变，而不是其他外泌体亚基基因突变体，也引起MT表型。RNA深度测序分析（RNA-seq）显示突变菌株中细胞周期和MT相关转录物水平的广泛变化。总的来说，本研究中提供的数据表明Dis 3在连接RNA代谢，MT和细胞周期进程中的进化保守作用。版权所有© 2011约翰威利父子有限公司.
Subunits of the RNA processing exosome assemble into structurally distinct protein complexes that function in disparate cellular compartments and RNA metabolic pathways. Here, in a genetic, cell biological and transcriptomic analysis, we examined the role of Dis3, an essential polypeptide with endo‐ and 3′ → 5′ exo‐ribonuclease activity, in cell cycle progression. We present several lines of evidence that perturbation of DIS3 affects microtubule (MT) localization and structure inSaccharomyces cerevisiae. Cells with a DIS3 mutant: (a) accumulate anaphase and pre‐anaphase mitotic spindles; (b) exhibit spindles that are misorientated and displaced from the bud neck; (c) harbour elongated spindle‐associated astral MTs; (d) have an increased G1astral MT length and number; and (e) are hypersensitive to MT poisons. Mutations in the core exosome genes RRP4 and MTR3 and the exosome cofactor gene MTR4, but not other exosome subunit gene mutants, also elicit MT phenotypes. RNA deep sequencing analysis (RNA‐seq) shows broad changes in the levels of cell cycle‐ and MT‐related transcripts in mutant strains. Collectively, the data presented in this study suggest an evolutionarily conserved role for Dis3 in linking RNA metabolism, MTs and cell cycle progression. Copyright © 2011 John Wiley & Sons, Ltd.