Stimulation of sperm respiration rates by speract and resact at alkaline extracellular pH.
Stimulation of sperm respiration rates by speract and resact at alkaline extracellular pH.
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speract 和 resact 在碱性细胞外 pH 值下刺激精子呼吸速率。
DOI：
10.1095/biolreprod30.5.1167
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发表时间：
1984
影响因子：
3.6
通讯作者：
Garbers,DL
中科院分区：
文献类型：
--
作者：
Suzuki,N;Garbers,DL
在胞外pH为6.6时，从点状阿巴克卵冻中分离出的一种多肽(Resact)能提高点状阿巴克精子的呼吸频率，但不能激活石龙子的精子。反之，精氨酸(Gly-Phe-Asp-Leu-Asn-Gly-Val-Gly)可提高L。图为，但不是点状精虫。在正常的海水pH值(7.6-8.0)下，马尾藻的蛋冻，或毫升的蛋冻。Pigtus纯化后不含精子，抑制L。图为精子的呼吸率。类似地，蛋冻也可以从果冻中提取。PICTUS对马尾藻精子的呼吸速率有抑制作用。抑制呼吸的凝胶成分是不可透析性的。果冻对呼吸的抑制作用可被精液所逆转。通过添加对点状精子的反应物，可以获得精液。Speract刺激L。在异源或同源(无精子)凝胶存在的情况下，精子呼吸在约1海里处达到半最大值。莫能菌素A是一种提高精子细胞内pH值的离子载体，它逆转了果冻对呼吸的抑制。这些结果表明，面对卵冻中存在的抑制成分，与卵子相关的两种多肽(SERACT和REACT)可以刺激精子的运动和代谢。此外，这些多肽还表现出物种特异性。
At an extracellular pH of 6.6, a peptide (resact) isolated from the egg jelly ofArbacia punctulataincreased the respiration rates ofA.punctulataspermatozoa but did not activate sperm cells fromLytechinus pictus. In contrast, speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly), elevated the respiration rates ofL. pictusbut notA.punctulataspermatozoa. At normal seawater pH (7.6–8.0) egg jelly fromA.punctulata, or egg jelly fromL. pictuspurified free of speract, inhibitedL. pictussperm respiration rates. Similarly, the egg jelly fromL. pictusinhibited the respiration rates ofA.punctulataspermatozoa. The jelly component responsible for the inhibition of respiration was nondialyzable. The inhibition of respiration induced by jelly could be reversed by the addition of speract toL. pictusspermatozoa and by the addition of resact toA.punctulataspermatozoa. Speract stimulatedL. pictussperm respiration half-maximally at about 1 nM in the presence of either heterologous or homologous (speract-free) jelly. Monensin A, an ionophore which elevates sperm intracellular pH, reversed the jelly inhibition of respiration. These results demonstrate that two peptides associated with eggs (speract and resact) can stimulate sperm motility and metabolism in the face of inhibitory components present in the egg jelly. Additionally, the peptides demonstrate species specificity.