Expression of mitochondria-related genes is elevated in overfeeding-induced goose fatty liver
Expression of mitochondria-related genes is elevated in overfeeding-induced goose fatty liver
复制标题
过度饲喂诱发鹅脂肪肝线粒体相关基因表达升高
DOI：
10.1016/j.cbpb.2015.11.006
复制
发表时间：
2016-02-01
影响因子：
2.2
通讯作者：
Geng, Tuoyu
中科院分区：
文献类型：
--
作者：
Osman, Rashid H.;Shao, Dan;Geng, Tuoyu
线粒体是细胞的动力库，是参与能量稳态的重要细胞器。线粒体质量和功能的改变可能导致代谢紊乱。以往的研究表明，线粒体质量丢失和功能障碍与人类和小鼠的非酒精性脂肪性肝病(NAFLD)有关。然而，目前尚不清楚线粒体基因是否参与鹅脂肪肝的发生发展。为了解决这个问题，我们确定了鹅线粒体基因对过度喂养和其他脂肪肝相关因素(例如，高胰岛素血症、高血糖和高脂血症)的反应。我们首先使用RNA-seq技术来确定正常饲喂和过度饲喂的鹅肝脏中的差异表达基因，然后进行生物信息学分析和定量PCR验证。数据表明，肝脏中的大多数线粒体基因都是由过度摄食诱导的。为了了解这些基因是如何在脂肪肝的背景下调节的，我们用高水平的葡萄糖、脂肪酸和胰岛素处理鹅原代肝细胞。结果表明，这些因素对线粒体相关基因的表达有一定的影响。综上所述，这些发现表明，过量饲养诱导线粒体基因表达是鹅脂肪肝发生发展所必需的，这种诱导部分归因于高血糖、高脂血症和高胰岛素血症。(C)2015 Elsevier Inc.保留所有权利。
Mitochondrion, the power house of the cell, is an important organelle involving in energy homeostasis. Change in mitochondrial mass and function may lead to metabolic disorders. Previous studies indicate that mitochondrial mass loss and dysfunction are associated with non-alcoholic fatty liver disease (NAFLD) in human and mouse. However, it is unclear whether mitochondrial genes are involved in the development of goose fatty liver. To address this, we determined the response of goose mitochondrial genes to overfeeding and other fatty liver related factors (e.g., hyperinsulinemia, hyperglycemia, and hyperlipidemia). We first employed RNA-seq technology to determine the differentially expressed genes in the livers from normally-fed vs. overfed geese, followed by bioinformatics analysis and quantitative PCR validation. Data indicated that a majority of mitochondrial genes in the liver were induced by overfeeding. To understand how these genes are regulated in the context of fatty liver, we treated goose primary hepatocytes with high levels of glucose, fatty acids and insulin. The results indicated that these factors had an influence on the expression of some mitochondria related genes. Together, these findings suggest that the induction of mitochondrial gene expression by overfeeding is required for the development of goose fatty liver, and this induction is partially attributable to hyperglycemia, hyperlipidemia and hyperinsulinemia. (C) 2015 Elsevier Inc. All rights reserved.