Enigma Plays Roles in Survival of Thyroid Carcinoma Cells through PI3K/AKT Signaling and Survivin
Enigma Plays Roles in Survival of Thyroid Carcinoma Cells through PI3K/AKT Signaling and Survivin
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DOI：
10.21873/anticanres.12623
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发表时间：
2018-06-01
影响因子：
2
通讯作者：
Lee, Seong Jin
中科院分区：
文献类型：
--
作者：
Kim, Yoon Jung;Hwang, Hyo-Jeong;Lee, Seong Jin
背景/目的：本研究的目的是评估enigma蛋白在甲状腺癌细胞存活中的作用。材料和方法：BCPAP和8505 C人甲状腺癌细胞。采用CCK-8法测定细胞活力，台盼蓝法测定死亡细胞百分比，细胞毒性法测定细胞毒活性，伤口愈合法测定细胞生长速率和细胞迁移。结果：在enigma siRNA转染的细胞中，细胞活力以及AKT和Survivin蛋白水平降低。死亡细胞百分比、细胞毒活性和裂解的聚（ADP-核糖）聚合酶（PARP）蛋白水平增加。转染p110 a质粒后，细胞活力、死亡细胞百分比、细胞毒活性以及AKT、存活素和裂解的PARP蛋白水平的改变被消除。随着基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）蛋白水平的降低以及p53和p21蛋白水平的升高，细胞生长速率和细胞迁移率降低。结论：Enigma通过调节磷脂酰肌醇-3激酶/AKT信号通路和Survivin影响细胞存活，并通过MMP-2、MMP-9、p53和p21参与甲状腺癌细胞的增殖和迁移。
Background/Aim: The aim of the present study was to assess the role of enigma protein in survival of thyroid carcinoma cells. Materials and Methods: BCPAP and 8505C human thyroid carcinoma cells were used. Cell viability using CCK-8 assay, the percentage of dead cells using trypan blue assay, cytotoxic activity using cytotoxicity assay, cell growth rate and cell migration using wound-healing assay were performed. Results: In enigma siRNA-transfected cells, cell viability, and the protein levels of AKT and survivin decreased. The percentage of dead cells, cytotoxic activity and cleaved poly (ADP-ribose) polymerase (PARP) protein levels increased. After transfection of p110a plasmid, the alterations in cell viability, the percentage of dead cells, cytotoxic activity, and protein levels of AKT, survivin and cleaved PARP were abrogated. Cell growth rate and cell migration were reduced with reduction of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) protein levels, as well as increased p53 and p21 protein levels. Conclusion: Enigma affects cell survival through modulation of phosphatidylinositol-3 kinase/AKT signaling and survivin, and regulates cell proliferation and migration via involvement of MMP-2, MMP-9, p53 and p21 in thyroid carcinoma cells.