Equilibrium between Inactive Ready Ni-SIr and Active Ni-SIa States of [NiFe] Hydrogenase Studied by Utilizing Ni-SIr-to-Ni-SIa Photoactivation
Equilibrium between Inactive Ready Ni-SIr and Active Ni-SIa States of [NiFe] Hydrogenase Studied by Utilizing Ni-SIr-to-Ni-SIa Photoactivation
复制标题
利用 Ni-SIr 至 Ni-SIa 光活化研究 [NiFe] 氢化酶的非活性就绪 Ni-SIr 和活性 Ni-SIa 状态之间的平衡
DOI：
10.1039/c7cc06061k
复制
发表时间：
2017
影响因子：
4.9
通讯作者：
and Shun Hirota
中科院分区：
文献类型：
--
作者：
Hulin Tai;Liang Xu;Koji Nishikawa;Yoshiki Higuchi;and Shun Hirota
此前，发现[NiFe]氢化酶的Ni-SIr态通过光照射转化为Ni-SIa态。在此，在Ni-SIr-to-Ni-SIr光活化后，Ni-SIa态重新转变为Ni-SIr态获得了大的活化能和大的动力学同位素效应，这表明Ni-SIa态与H2O反应并为Ni-SIr态留下桥接羟基配体。
Previously, the Ni-SIr state of [NiFe] hydrogenase was found to convert to the Ni-SIa state by light irradiation. Herein, large activation energies and a large kinetic isotope effect were obtained for the reconversion of the Ni-SIa state to the Ni-SIr state after the Ni-SIr-to-Ni-SIa photoactivation, suggesting that the Ni-SIa state reacts with H2O and leaves a bridging hydroxo ligand for the Ni-SIr state.