Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture
Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture
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DOI：
10.1364/boe.415555
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发表时间：
2021-06-01
影响因子：
3.4
通讯作者：
Pavone, Francesco Saverio
中科院分区：
文献类型：
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作者：
Costantini, Irene;Mazzamuto, Giacomo;Pavone, Francesco Saverio
虽然人脑切片上的神经元密度分析可从体视学研究中获得，但关于神经元在3D中的空间分布的数据仍然缺失。由于大脑皮层中的神经元组织非常不均匀，因此将所有神经元映射到给定体积中而不是依赖于稀疏采样方法至关重要。为了实现这一目标，我们实施了一种新的组织转换协议，以清除和标记人脑组织，我们利用双光子荧光显微镜的高分辨率光学切片进行三维介观重建。我们对100 mm3人脑样本进行神经元映射，并评估来自不同受试者（年轻人，成年人和老年人，健康和病理）的皮层各区域神经元的体积和密度分布。密度的定量评价结合样本内鉴定的数千个神经元的平均体积，使我们能够确定大脑结构的层特异性组织。(C)根据OSA开放获取出版协议的条款，2021年美国光学学会
Although neuronal density analysis on human brain slices is available from stereological studies, data on the spatial distribution of neurons in 3D are still missing. Since the neuronal organization is very inhomogeneous in the cerebral cortex, it is critical to map all neurons in a given volume rather than relying on sparse sampling methods. To achieve this goal, we implement a new tissue transformation protocol to clear and label human brain tissues and we exploit the high-resolution optical sectioning of two-photon fluorescence microscopy to perform 3D mesoscopic reconstruction. We perform neuronal mapping of 100mm3 human brain samples and evaluate the volume and density distribution of neurons from various areas of the cortex originating from different subjects (young, adult, and elderly, both healthy and pathological). The quantitative evaluation of the density in combination with the mean volume of the thousands of neurons identified within the specimens, allow us to determine the layer-specific organization of the cerebral architecture. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement