Spatial and temporal traction response in human airway smooth muscle cells.
Spatial and temporal traction response in human airway smooth muscle cells.
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DOI：
10.1152/ajpcell.00169.2002
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发表时间：
2002-10
期刊：
影响因子：
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通讯作者：
I. Tolic-Nørrelykke;J. Butler;Jianxin Chen;Ning Wang
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文献类型：
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作者：
I. Tolic-Nørrelykke;J. Butler;Jianxin Chen;Ning Wang
细胞对基质施加的牵引力在细胞伸展、迁移和收缩中是必不可少的。这些牵引力可以通过将细胞接种在柔性凝胶上并通过使用嵌入凝胶表面正下方的荧光珠测量凝胶的变形来确定。在这篇文章中，我们描述了图像相关方法（ICM）优化确定下收缩细胞的凝胶位移场。为了从位移场计算牵引场，我们使用最近开发的傅里叶变换牵引细胞术（FTTC）的方法。ICM和FTTC方法在用收缩激动剂组胺或舒张激动剂异丙肾上腺素刺激期间应用于人气道平滑肌细胞。组胺激活后，细胞收缩的总体强度（中位牵引强度、从细胞转移到凝胶的能量和净收缩力矩）增加，而异丙肾上腺素处理后则减少。细胞表现出区域差异的时间过程中的牵引治疗。组胺引起的牵引力增加的时间演变和幅度在不同的细胞突起之间有显着差异，而核区域的反应最小。这些结果表明，细胞粘附和收缩的细胞内介质在细胞的不同区域以不同的速率和强度响应于收缩刺激。
Tractions that cells exert on their substrates are essential in cell spreading, migration, and contraction. These tractions can be determined by plating the cells on a flexible gel and measuring the deformation of the gel by using fluorescent beads embedded just below the surface of the gel. In this article we describe the image correlation method (ICM) optimized for determining the displacement field of the gel under a contracting cell. For the calculation of the traction field from the displacement field we use the recently developed method of Fourier transform traction cytometry (FTTC). The ICM and FTTC methods are applied to human airway smooth muscle cells during stimulation with the contractile agonist histamine or the relaxing agonist isoproterenol. The overall intensity of the cell contraction (the median traction magnitude, the energy transferred from the cell to the gel, and the net contractile moment) increased after activation with histamine, and decreased after treatment with isoproterenol. Cells exhibited regional differences in the time course of traction during the treatment. Both temporal evolution and magnitude of traction increase induced by histamine varied markedly among different cell protrusions, whereas the nuclear region showed the smallest response. These results suggest that intracellular mediators of cell adhesion and contraction respond to contractile stimuli with different rates and intensities in different regions of the cell.