ESSENTIAL ROLE OF SMALL, ACID-SOLUBLE SPORE PROTEINS IN RESISTANCE OF BACILLUS-SUBTILIS SPORES TO UV-LIGHT
ESSENTIAL ROLE OF SMALL, ACID-SOLUBLE SPORE PROTEINS IN RESISTANCE OF BACILLUS-SUBTILIS SPORES TO UV-LIGHT
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DOI：
10.1128/jb.167.1.174-178.1986
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发表时间：
1986-07-01
影响因子：
3.2
通讯作者：
SETLOW, P
中科院分区：
文献类型：
--
作者：
MASON, JM;SETLOW, P
构建了在编码一种或两种主要小酸溶性孢子蛋白（SASP；称为SASP-α和SASP-β）的基因中含有缺失的枯草芽孢杆菌菌株。这些突变体正常形成孢子，但孢子缺乏 SASP-α、SASP-β 或两种蛋白质。在这些突变体中，次要SASP的水平没有增加，但SASP-.alpha的水平却增加了。 SASP-β-突变体中的SASP-β-突变体增加了约两倍，并且SASP-β的水平增加了约两倍。在SASP-α-突变体中增加约两倍。缺失菌株的生长速率与野生型菌株在丰富或贫乏生长培养基中的生长速率相同，孢子萌发的起始也是如此。然而，在所有测试的培养基中，SASP-α-、-β-菌株的孢子生长明显慢于野生型孢子。 SASP-β-孢子的耐热性与野生型孢子相同，但略高于SASP-α-和SASP-α--β-孢子。然而，SASP-α-和SASP-α-β-孢子比营养细胞耐热得多。 SASP-β-和野生型孢子的紫外线抗性也相同。然而，SASP-α-β-孢子比野生型或SASP-α--β-菌株的对数期细胞对紫外线稍微敏感（后者具有相同的紫外线抗性）； SASP-.α.-孢子比SASP-.α.--.β.-孢子稍微更耐紫外线。这些数据强烈表明SASP，特别是SASP-α，与枯草芽孢杆菌孢子的UV光抗性有关。
Bacillus subtilis strains containing deletions in the genes coding for one or two of the major small, acid-soluble spore proteins (SASP; termed SASP-.alpha. and SASP-.beta.) were constructed. These mutants sporulated normally, but the spores lacked either SASP-.alpha., SASP-.beta., or both proteins. The level of minor SASP did not increase in these mutants, but the level of SASP-.alpha. increased about twofold in the SASP-.beta.- mutant, and the level of SASP-.beta. increased about twofold in the SASP-.alpha.- mutant. The growth rates of the deletion strains were identical to that of the wild-type strain in rich or poor growth media, as was the initiation of spore germination. However, outgrowth of spores of the SASP-.alpha.-,-.beta.- strain was significantly slower than that of wild-type spores in all media tested. The heat resistance of SASP-.beta.- spores was identical to that of wild-type spores but slightly greater than that of SASP-.alpha.- and SASP-.alpha.--.beta.- spores. However, the SASP-.alpha.- and SASP-.alpha.--.beta.- spores were much more heat resistant than vegetative cells. The UV light resistances of SASP-.beta.- and wild-type spores were also identical. However, SASP-.alpha.--.beta.- spores were slightly more sensitive to UV light than were log-phase cells of the wild-type or SASP-.alpha.--.beta.- strain (the latter have identical UV light resistances); SASP-.alpha.- spores were slightly more UV light resistant than SASP-.alpha.--.beta.- spores. These data strongly implicate SASP, in particular SASP-.alpha., in the UV light resistance of B. subtilis spores.