Crucial factors of the light perception machinery and their impact on growth and cellulase gene transcription in Trichoderma reesei
Crucial factors of the light perception machinery and their impact on growth and cellulase gene transcription in Trichoderma reesei
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DOI：
10.1016/j.fgb.2010.02.001
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Ulises Esquivel-Naranjo, Edgardo
中科院分区：
文献类型：
--
作者：
Castellanos, Francisco;Schmoll, Monika;Ulises Esquivel-Naranjo, Edgardo
在里氏木霉中，光刺激纤维素酶基因的转录，这种调节至少部分是通过蛋白质使者发生的。在这里，我们分析了BLR光受体复合体(BLR1/BLR2)在光生孢子形成和基因表达调控中的作用。这两种反应都依赖于两种BLR蛋白。对Delta blr1、abr2和Delta env1突变体的分析表明，BLR蛋白在光照下调节生长。对env1突变菌株的分析表明，特使允许真菌耐受持续的光暴露，抑制了木霉感知光强变化的能力，并表明它参与了负调控反馈。它作为抑制因子的活动建立了一段对第二次光治疗不敏感的时期。有趣的是，光刺激纤维素酶基因的表达也受到blr1和blr2的调节，这表明BLR蛋白在这一途径中起着关键作用。(C)2010 Elsevier Inc.保留所有权利。
In Trichoderma reesei light stimulates transcription of cellulase genes and this regulation has been found to occur, at least in part, through the protein ENVOY. Here we analyzed the role of the BLR photoreceptor complex (BLR1/BLR2) in photoconidiation and the regulation of gene expression. Both responses were dependent on both BLR proteins. Analyses of Delta blr1, Ablr2 and Delta env1 mutants showed that the BLR proteins regulate growth under illumination. Analysis of env1 mutant strains indicated that ENVOY allows the fungus to tolerate continuous exposure to light, damped the capacity of Trichoderma to perceive changes in light intensity, and suggested that it participates in a negative regulatory feedback. Its activity as repressor establishes a period of insensitivity to a second light treatment. Interestingly, the stimulation of cellulase gene expression by light was also modulated by both blr1 and blr2, indicating a key role of the BLR proteins in this pathway. (C) 2010 Elsevier Inc. All rights reserved.