Blocking Glutamate Receptors in the Waist Area of the Parabrachial Nucleus Decreases Taste Reactivity Behaviors in Conscious Rats
Blocking Glutamate Receptors in the Waist Area of the Parabrachial Nucleus Decreases Taste Reactivity Behaviors in Conscious Rats
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DOI：
10.1093/chemse/bjn081
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发表时间：
2009-03-01
期刊：
影响因子：
3.5
通讯作者：
King, Michael S.
中科院分区：
文献类型：
--
作者：
Biondolillo, Joseph W.;Williams, Learnel A.;King, Michael S.
臂旁核的“腰部”区域 (W) 包含接收口腔感觉输入并在口腔运动行为启动中发挥作用的神经元。免疫组织化学数据表明，W 中的神经元接受谷氨酸输入并表达谷氨酸受体，但 W 中谷氨酸神经传递的行为作用尚未得到研究。为了确定特定谷氨酸受体在味觉反应行为中的作用，在口内输注 0.1 M 氯化钠、0.1 M 蔗糖、0.03 M 盐酸和 0.003 M 盐酸奎宁期间，通过反向微透析将谷氨酸受体阻滞剂递送到 W 中。与基线水平相比，用 6-氰基-7-硝基喹喔啉-2,3-二酮二钠 (CNQX) 阻断 W 中的 α-氨基-3-羟基-5-甲基-异恶唑丙酸酯 (AMPA)/红藻氨酸离子型谷氨酸受体，可将每种促味剂的摄食味觉反应行为降低 72-85%（P < 0.05）。阻断 N-甲基-D-天冬氨酸受体以及 1 型和 III 类代谢型谷氨酸受体对促味剂的味觉反应性反应影响较小。这些数据为清醒大鼠 W 中谷氨酸能神经传递的行为作用提供了有力的证据。
The "waist" area (W) of the parabrachial nucleus contains neurons that receive orosensory input and play a role in the initiation of oromotor behaviors. Immunohistochemical data indicate that neurons in W receive glutamatergic input and express glutamate receptors, but a behavioral role for glutamate neurotransmission within W has not been investigated. To determine the role of specific glutamate receptors in taste reactivity behaviors, glutamate receptor blockers were delivered into W by reverse microdialysis during intraoral infusion of 0.1 M sodium chloride, 0.1 M sucrose, 0.03 M hydrochloric acid, and 0.003 M quinine hydrochloride. Blocking alpha-amino-3-hydroxy-5-methyl-isoxazolepropionate (AMPA)/kainate ionotropic glutamate receptors in W with 6-cyano-7-nitroquinoxaline-2,3-dione disodium (CNQX) reduced ingestive taste reactivity behaviors to each tastant by 72-85% compared with baseline levels (P's < 0.05). Blocking N-methyl-D-aspartate receptors as well as type 1 and group III metabotropic glutamate receptors had minor effects on taste reactivity responses to the tastants. These data provide strong evidence for a behavioral role of glutamatergic neurotransmission in W in conscious rats.