Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia
Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia
复制标题
DOI：
10.1007/s00418-012-0991-7
复制
发表时间：
2012-07
影响因子：
2.3
通讯作者：
K. Kopylow;H. Staege;W. Schulze;H. Will;C. Kirchhoff
中科院分区：
文献类型：
--
作者：
K. Kopylow;H. Staege;W. Schulze;H. Will;C. Kirchhoff
人精原细胞（Spg）及其胎儿前体细胞表达成纤维细胞生长因子受体3（FGFR 3）。为了进一步阐明FGFR 3在控制Spg自我更新、增殖和/或分化中的作用，并缩小正常成年人睾丸中FGFR 3阳性细胞类型，结合免疫荧光和共聚焦荧光显微镜分析生精小管的组织切片和整体标本。FGFR 3蛋白主要在A型Spg亚群的细胞膜和细胞质囊泡中观察到，该亚群包括含有染色质稀疏区的Adark型。FGFR 3的胞浆表达和增殖相关抗原KI-67的核表达是相互排斥的。同样，FGFR 3阳性的Spg不表达DoublemexandMab-3相关转录因子1（DMRT 1）。相比之下，未分化胚胎细胞转录因子1（UTF 1）和卵巢癌生存时间相关PHD指蛋白1（SPOC 1）在FGFR 3阳性的Spg细胞核中共表达。整个安装的曲细精管说明了FGFR 3/UTF 1双阳性Spg的克隆形成排列，其主要以成对或四联体形式出现，并且与KIT阳性Spg不同，与KI-67标记的簇没有重叠。总之，在成年人睾丸中，FGFR 3表达是很少分裂的A型Spg的小克隆的特征，其类似于“未分化的”Spg，包括精原干细胞。
Human spermatogonia (Spg) and their fetal precursors express fibroblast growth factor receptor 3 (FGFR3). To further elucidate the role of FGFR3 in the control of Spg self-renewal, proliferation, and/or differentiation, and to narrow down the FGFR3-positive cell type(s) in the normal adult human testis, tissue sections and whole mount preparations of seminiferous tubules were analyzed combining immunofluorescence and confocal fluorescence microscopy. FGFR3 protein was chiefly observed in cellular membranes and cytoplasmic vesicles of a subpopulation of type A Spg, which comprised the chromatin rarefaction zone-containing type Adark. Cytoplasmic expression of FGFR3 and nuclear expression of proliferation-associated antigen KI-67 were mutually exclusive. Similarly, FGFR3-positive Spg were negative forDoublesexandMab-3related transcription factor 1 (DMRT1). By contrast, undifferentiated embryonic cell transcription factor 1 (UTF1) and survival time-associated PHD finger in ovarian cancer 1 protein (SPOC1) were co-expressed in the nuclei of FGFR3-positive Spg. Whole mounted seminiferous tubules illustrated the clonogenic arrangement of the FGFR3/UTF1 double-positive Spg, which mainly occurred as pairs or quadruplets and, different from the KIT-positive Spg, showed no overlap with KI-67 labeled clusters. Taken together, in the adult human testis, FGFR3 expression is a feature of small clones of rarely dividing type A Spg which resemble “undifferentiated” Spg, including the spermatogonial stem cells.