Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells
Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells
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DOI：
10.1371/journal.pone.0210060
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发表时间：
2019-01-02
期刊：
影响因子：
3.7
通讯作者：
Ema, Masatsugu
中科院分区：
文献类型：
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作者：
Watanabe, Chisato;Matsushita, Jun;Ema, Masatsugu
血管内皮生长因子受体3（vascular endothelial growth factor receptor 3，Vegfr 3）作为淋巴管内皮细胞和血管内皮细胞的标志物，在正常和病理条件下研究血管生成和淋巴管生成具有重要价值。在这里，我们报告了一种新的转基因（Tg）小鼠，表达膜定位的荧光报告蛋白，Gap 43-金星，Vegfr 3调控序列的控制下的一代。Vegfr 3-Gap 43-Venus BAC Tg重现了胚胎发育和肿瘤发育期间血管和淋巴管内皮细胞中的内源性Vegfr 3表达。因此，该Tg小鼠系有助于在生理和病理背景下研究血管生成和淋巴管生成的有价值的模型。
Vascular endothelial growth factor receptor 3 (Vegfr3) has been widely used as a marker for lymphatic and vascular endothelial cells during mouse embryonic development and in adult mouse, making it valuable for studying angiogenesis and lymphangiogenesis under normal and pathological conditions. Here, we report the generation of a novel transgenic (Tg) mouse that expresses a membrane-localized fluorescent reporter protein, Gap43-Venus, under the control of the Vegfr3 regulatory sequence. Vegfr3-Gap43-Venus BAC Tg recapitulated endogenous Vegfr3 expression in vascular and lymphatic endothelial cells during embryonic development and tumor development. Thus, this Tg mouse line contributes a valuable model to study angiogenesis and lymphangiogenesis in physiological and pathological contexts.