INFLUENCE OF HYDROGEN-CONSUMING BACTERIA ON CELLULOSE DEGRADATION BY ANAEROBIC FUNGI
INFLUENCE OF HYDROGEN-CONSUMING BACTERIA ON CELLULOSE DEGRADATION BY ANAEROBIC FUNGI
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DOI：
10.1128/aem.56.12.3793-3797.1990
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发表时间：
1990-12-01
影响因子：
4.4
通讯作者：
PRINS, RA
中科院分区：
文献类型：
--
作者：
MARVINSIKKEMA, FD;RICHARDSON, AJ;PRINS, RA
在几种厌氧真菌的培养中，产甲烷菌嗜铬甲烷杆菌、布氏甲烷杆菌或史密斯甲烷短杆菌的存在使纤维素发酵水平提高了5%至10%。当新天牛Neocallimastix sp.菌株L2与产甲烷菌共培养，其纤维素发酵速率也较纯培养有所提高。产甲烷菌导致发酵产物转变为更多的醋酸盐，而较少的乳酸、琥珀酸和乙醇。在厌氧真菌和史密斯分枝杆菌共培养中，甲酸盐的转移不会进一步刺激超过H2转移水平的纤维素酶的分解。当瘤胃单胞菌作为一种耗氢微生物与新卡莱姆斯蒂亚种共培养时。菌株L2的纤维素酶降解速率和水平均有所提高。观察到的产甲烷菌的影响被解释为表明电子从电子汇碳产物的形成转移到通过还原的吡啶核苷酸的氢，有利于额外的乙酸酯的产生，可能还有三磷酸腺苷。反胃沙门氏菌是如何发挥其影响的尚不清楚。这可能是由于真菌降低了电子汇产物的产量，可能是由于反刍链霉菌消耗电子汇产物或氢，或者是由于竞争在纯培养条件下对纤维分解产生抑制作用的游离糖所致。
The presence of methanogens Methanobacterium arboriphilus, Methanobacterium bryantii, or Methanobrevibacter smithii increased the level of cellulose fermentation by 5 to 10% in cultures of several genera of anaerobic fungi. When Neocallimastix sp. strain L2 was grown in coculture with methanogens the rate of cellulose fermentation also increased relative to that for pure cultures of the fungus. Methanogens caused a shift in the fermentation products to more acetate and less lactate, succinate, and ethanol. Formate transfer in cocultures of anaerobic fungi and M. smithii did not result in further stimulation of cellulolysis above the level caused by H2 transfer. When Selenomonas ruminantium was used as a H2-consuming organism in coculture with Neocallimastix sp. strain L2, both the rate and level of cellulolysis increased. The observed influence of the presence of methanogens is interpreted to indicate a shift of electrons from the formation of electron sink carbon products to H2 via reduced pyridine nucleotides, favoring the production of additional acetate and probably ATP. It is not known how S. ruminantium exerts its influence. It might result from a lowered production of electron sink products by the fungus, from consumption of electron sink products or H2 by S. ruminantium, or from competition for free sugars which in pure culture could exert an inhibiting effect on cellulolysis.