Effects of glucose on biochemical immune responses and hepatic gene expression in common carp, Cyprinus carpio L
Effects of glucose on biochemical immune responses and hepatic gene expression in common carp, Cyprinus carpio L
复制标题
葡萄糖对鲤鱼生化免疫反应和肝脏基因表达的影响
DOI：
10.1080/13102818.2018.1534554
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发表时间：
2018-11-02
影响因子：
1.4
通讯作者：
Yu，Juhua
中科院分区：
文献类型：
--
作者：
Yu，Fan;Li，Jianlin;Yu，Juhua
摘要葡萄糖代谢是动物体内重要的生物学过程，它与免疫反应和疾病等多种生理活动密切相关。本研究通过观察葡萄糖注射液对小鼠血清指标和肝脏基因表达的影响，探讨葡萄糖注射液对小鼠的免疫应答作用。我们对鲤鱼肝脏进行了血清分析和高通量测序分析。葡萄糖注射液可上调血清GLU和白蛋白水平。转录组分析显示，GO分类中对外部刺激的应答类别、KEGG分析中的PPAR-signaling途径、补体和凝血级联途径富集在葡萄糖注射组和对照组之间差异表达的基因中。在这些途径中，氧化还原调节蛋白FAM 213 A样，细胞外超氧化物歧化酶[Cu-Zn]样和推定的天然抗性相关巨噬细胞蛋白（NRAMP）上调葡萄糖刺激。25-羟维生素D-1 α羟化酶、维生素D 25-羟化酶和natterin-3-like被葡萄糖注射液显著下调。本研究首次报道了鲤鱼血清和肝脏转录组对血清葡萄糖升高的反应。所获得的数据为未来理解能量代谢和免疫系统之间的联系提供了有价值的线索。
Abstract Glucose metabolism is a key biological process in animals, as it is related to multiple activities including immune response and diseases. In this study, we focused on serum parameter changes and hepatic gene expression to explore the immune response to glucose injection. We performed serum analysis and high-throughput sequencing analysis in the liver of common carp (Cyprinus carpio L). The serum GLU and albumin were up-regulated by glucose injection. The transcriptome analysis showed that the categories of response to external stimulus in GO category, PPAR-signalling pathway, complement and coagulation cascades pathways in KEGG analysis were enriched in genes that were differently expressed between the glucose injection group and the control group. Among these pathways, redox-regulatory protein FAM213A-like, extracellular superoxide dismutase [Cu–Zn]-like and putative natural resistance-associated macrophage protein (NRAMP) were up-regulated by glucose stimulation. The 25-hydroxyvitamin D-1 alpha hydroxylase, vitamin D 25-hydroxylase and natterin-3-like were significantly down-regulated by glucose injection. The present study is the first report of serum and hepatic transcriptome changes in common carp in response to glucose increase in serum. The obtained data provide valuable clues for future efforts into understanding the connection between the energy metabolism and the immune system.