Analysis of cocaine and cocaine metabolites by high pressure liquid chromatography.
Analysis of cocaine and cocaine metabolites by high pressure liquid chromatography.
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通过高压液相色谱法分析可卡因和可卡因代谢物。
DOI：
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发表时间：
1980
影响因子：
2.5
通讯作者：
T. Morarity
中科院分区：
文献类型：
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作者：
M. Evans;T. Morarity
开发了高压液相色谱程序用于分析生物体液中的可卡因和两种代谢物（去甲可卡因和苯甲酰爱康宁）。用氯仿/异丙醇(3/2)萃取后，用mu-Bondapak C-18反相柱进行色谱法，用水/乙腈/甲醇(8/1/1)(含有1％乙酸和0.3M EDTA)洗脱。在 235 nm 处对各个化合物进行紫外检测，并根据与内标的峰高比进行定量。基于 0.5 mL 样品的检测限小于 1 微克/mL。通过对洗脱峰进行直接探针质谱分析并与已知标准品进行比较来确认各个化合物。
A high pressure liquid chromatography procedure was developed for analysis of cocaine and two metabolites (norcocaine and benzoylecgonine) in biological fluid. Following extraction with chloroform/Isopropanol (3/2), chromatography is performed with a mu-Bondapak C-18 reverse phase column eluted with water/acetonitrile/methanol (8/1/1) containing 1% acetic acid and 0.3 M EDTA. Ultraviolet detection of individual compounds was made at 235 nm and quantitation was based on peak height ratio to internal standard. Detection limit based on 0.5 mL of sample is less than 1 microgram/mL. Confirmation of the individual compounds was made by direct probe mass spectral analysis of the eluted peaks and comparison to known standards.