Microcystins distribution, bioaccumulation, and Microcystis genotype succession in a fish culture pond
Microcystins distribution, bioaccumulation, and Microcystis genotype succession in a fish culture pond
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养鱼池中微囊藻毒素的分布、生物累积和微囊藻基因型演替
DOI：
10.1016/j.scitotenv.2019.06.156
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发表时间：
2019-10-20
影响因子：
9.8
通讯作者：
Wu, Miao
中科院分区：
文献类型：
--
作者：
Bi, Xiangdong;Dai, Wei;Wu, Miao
在淡水养殖池塘中，蓝藻水华和微囊藻毒素（MCs）污染因其毒性效应而引起人们的广泛关注。为深入了解养殖池塘中的蓝藻问题，于2017年5月至2017年11月对某养殖池塘中微囊藻的分布、生物积累和微囊藻基因型演替进行了调查。过滤水、悬浮物和沉积物中的MCs浓度分别为1.16 - 3.66 μ g/L、0.64 - 13.98 μ g/g DW和1.34 - 5.90 μ g/g DW。此外，化学需氧量与悬浮细胞浓度呈正相关。MCs在商品鱼各组织中的积累量大小为肝脏>肾脏>肠>肌肉。肌肉中的MC含量比世界卫生组织建议的每日耐受摄入量高出4.3倍。从653条微囊藻ITS序列中鉴定出24种基因型。在调查期间，观察到相当大的基因型变异和快速的基因型演替和优势基因型不存在。冗余度分析表明，微囊藻基因型可以显著影响悬浮物中微囊藻比例的变化，从而影响悬浮物中微囊藻的浓度。(C)2019爱思唯尔B. V.保留所有权利。
In freshwater aquaculture ponds, cyanobacterial blooms and microcystins (MCs) pollution have attracted considerable attention due to their toxic effects. To provide an insight into cyanobacterial problems in aquaculture ponds, MCs distribution, bioaccumulation, and Microcystis genotype succession in a fishpond were investigated from May 2017 to November 2017.The distribution of MCs in filtered water, seston, and sediment varied considerably among months. MCs concentrations in filtered water, seston, and sediment ranged from 1.16 to 3.66 mu g/L, 0.64 to 13.98 mu g/g DW, and 1.34 to 5.90 mu g/g DW, respectively. In addition, chemical oxygen demand was positively correlated with sestonic MCs concentrations. MCs concentrations accumulated in different tissues of market-size fish were in the order of liver > kidney > intestine > muscle. MCs content in muscle was 4.3 times higher than the WHO recommended tolerable daily intake level. Twenty-four ITS genotypes of Microcystis were identified from a total of 653 sequences. During the survey period, considerable genotype variation and rapid genotype succession were observed and dominant genotype was absent. A redundancy analysis revealed that Microcystis genotypes could significantly influence the variations in the proportions of the potentially toxic Microcystis, which could in turn influence the MCs concentrations in seston. (C) 2019 Elsevier B.V. All rights reserved.