Laser Cutting Eliminates Nucleic Acid Cross-Contamination in Dried-Blood-Spot Processing
Laser Cutting Eliminates Nucleic Acid Cross-Contamination in Dried-Blood-Spot Processing
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DOI：
10.1128/jcm.02549-12
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发表时间：
2012-12-01
影响因子：
9.4
通讯作者：
Coombs, Robert
中科院分区：
文献类型：
--
作者：
Murphy, Sean C.;Daza, Glenda;Coombs, Robert
干血斑（DBS）可用于分子测定，但易于因交叉污染而出现假阳性。在我们的疟疾DBS检测中，尽管采用了适用于HIV-1的清洁技术，但仍遇到了交叉污染。因此，我们开发了一种非接触式激光切割系统，有效地消除了DBS加工过程中的交叉污染。
Dried blood spots (DBS) are useful for molecular assays but are prone to false positives from cross-contamination. In our malaria DBS assay, cross-contamination was encountered despite cleaning techniques suitable for HIV-1. We therefore developed a contact-free laser cutting system that effectively eliminated cross-contamination during DBS processing.