Comparative analysis of complete chloroplast genome sequences of two tropical trees Machilus yunnanensis and Machilus balansae in the family Lauraceae.
Comparative analysis of complete chloroplast genome sequences of two tropical trees Machilus yunnanensis and Machilus balansae in the family Lauraceae.
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DOI：
10.3389/fpls.2015.00662
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发表时间：
2015
影响因子：
5.6
通讯作者：
Corlett RT
中科院分区：
文献类型：
--
作者：
Song Y;Dong W;Liu B;Xu C;Yao X;Gao J;Corlett RT
Malilus是一种大型(约100 sp.)樟科的一个乔木属，分布于热带和亚热带的东亚。这种形态一致的属的分子物种鉴定和系统发育研究都受到常用生物标志物中变异位点不足的限制。为了更好地了解楠属植物叶绿体基因组中的突变模式，对两个物种的完整叶绿体基因组进行了测序。云南楠和巴兰楠的质粒体长度分别为152、622和152、721个碱基。共鉴定出7个高变异区，准确定位了297个突变事件，包括CCSA-ndhD区的1个微倒位、65个INDELs和231个替换。共发现36个可用于物种鉴定的微卫星位点，在基因编码区发现95个单核苷酸突变。
Machilus is a large (c. 100 sp.) genus of trees in the family Lauraceae, distributed in tropical and subtropical East Asia. Both molecular species identification and phylogenetic studies of this morphologically uniform genus have been constrained by insufficient variable sites among frequently used biomarkers. To better understand the mutation patterns in the chloroplast genome of Machilus, the complete plastomes of two species were sequenced. The plastomes of Machilus yunnanensis and M. balansae were 152, 622 and 152, 721 bp, respectively. Seven highly variable regions between the two Machilus species were identified and 297 mutation events, including one micro-inversion in the ccsA-ndhD region, 65 indels, and 231 substitutions, were accurately located. Thirty-six microsatellite sites were found for use in species identification and 95 single-nucleotide changes were identified in gene coding regions.