Single-molecule detection of DNA via sequence-specific links between F1-ATPase motors and gold nanorod sensors.
Single-molecule detection of DNA via sequence-specific links between F1-ATPase motors and gold nanorod sensors.
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DOI：
10.1039/b716744j
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发表时间：
2008-02
期刊：
影响因子：
6.1
通讯作者：
J. York;D. Spetzler;Fusheng Xiong;W. Frasch
中科院分区：
文献类型：
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作者：
J. York;D. Spetzler;Fusheng Xiong;W. Frasch
我们报道了一种新型生物传感纳米器件的构建，用于检测单个序列特异性靶DNA分子。纳米器件的组装是通过单个3',5'-二生物素化的DNA分子将固定化的f1 - atp酶分子马达和功能化的金纳米棒结合在一起进行的。靶向依赖性3',5'-二生物素化DNA桥通过结合连接和脱核反应（LXR）形成，具有特异性，能够选择单核苷酸多态性（SNP）。利用暗场显微镜检测金纳米棒，对组装的纳米器件进行定量分析就足以区分出仅1800个DNA桥和非特异性结合的纳米棒的存在。当纳米棒通过DNA桥接时，f1 - atp酶的旋转机制可以驱动纳米棒旋转。因此，旋转将完全组装的器件与非特异性结合的纳米棒区分开来，导致灵敏度限制为一个zeptomole（600个分子）。
We report the construction of a novel biosensing nanodevice to detect single, sequence-specific target DNA molecules. Nanodevice assembly occurs through the association of an immobilized F1-ATPase molecular motor and a functionalized gold nanorod via a single 3',5'-dibiotinylated DNA molecule. Target-dependent 3',5'-dibiotinylated DNA bridges form by combining ligation and exonucleation reactions (LXR), with a specificity capable of selecting against a single nucleotide polymorphism (SNP). Using dark field microscopy to detect gold nanorods, quantitation of assembled nanodevices is sufficient to distinguish the presence of as few as 1800 DNA bridges from nonspecifically bound nanorods. The rotary mechanism of F1-ATPase can drive gold nanorod rotation when the nanorod is attached via the DNA bridge. Therefore, rotation discriminates fully assembled devices from nonspecifically bound nanorods, resulting in a sensitivity limit of one zeptomole (600 molecules).