Oxygen transport during hemodilution with a perfluorocarbon-based oxygen carrier: effect of altitude and hyperoxia.
Oxygen transport during hemodilution with a perfluorocarbon-based oxygen carrier: effect of altitude and hyperoxia.
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使用基于全氟化碳的氧载体进行血液稀释期间的氧气输送：海拔和高氧的影响。
DOI：
10.1152/japplphysiol.00152.2008
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Briceño,JuanCarlos
中科院分区：
文献类型：
--
作者：
Gardeazábal,Tatiana;Cabrera,Mariana;Cabrales,Pedro;Intaglietta,Marcos;Briceño,JuanCarlos
在海平面和海拔2，600 m处评价了使用全氟化碳氧载体（PFCOC）血液稀释后的氧输送和消耗。对15只麻醉大鼠分别进行40%循环血量的两次交换等容血液稀释。用胶体溶液进行第一次交换。第二次交换用80% PFCOC和20%胶体。然后用100%氧气对动物进行通气。实验在1.0 atm（海平面组，n= 9）或0.74 atm（2，600-m组，n= 6）的气压下进行。在基线和每次交换后15分钟测量血气、血细胞比容、荧光比容和血红蛋白含量。血液稀释后，两组中的总动脉含量均未被PFCOC改变。相比之下，两组的动静脉氧差显著增加，氧提取率也显著增加。在第二次交换中，虽然两组之间的总动脉含量相似，但在海平面上，全氟化碳和血浆相的贡献更大。动静脉氧差显着减少在海平面与较高的贡献，从全氟化碳和血浆阶段。总之，血液稀释与PFCOC诱导的氧输送和消耗的变化，不同的海拔高度。2，600-m组表现出较高的氧摄取率和动静脉氧差，氟碳化合物和血浆相的氧输送和卸载减少。因此，在海拔2，600米处，全氟辛烷磺酸的效力会降低，在使用全氟辛烷磺酸时必须考虑海拔高度。
Oxygen delivery and consumption after hemodilution with a perfluorocarbon-based oxygen carrier (PFCOC) was evaluated at sea level and at 2,600 m above sea level. Fifteen anesthetized rats were subjected to a two-exchange normovolemic hemodilution of 40% of the circulating blood volume each. First exchange was performed with a colloid solution. Second exchange was with 80% PFCOC and 20% colloid. Animals were then ventilated with 100% oxygen. Experiments were performed at barometric pressure of 1.0 atm (sea-level group,n= 9) or 0.74 atm (2,600-m group,n= 6). Blood gases, hematocrit, fluorocrit, and hemoglobin content were measured at baseline and 15 min after each exchange. After hemodilution, total arterial content was not modified by the PFCOC in either group. In contrast, arteriovenous oxygen difference increased significantly in both groups, as did the oxygen extraction ratio. In the second exchange, although total arterial content was similar between the two groups, the perfluorocarbon and plasma phases contributed significantly more at sea level. Arteriovenous oxygen difference was significantly less at sea level with a higher contribution from the perfluorocarbon and plasma phases. In conclusion, hemodilution with a PFCOC induced changes in oxygen delivery and consumption that differ with altitude. The 2,600-m group exhibited a higher oxygen extraction ratio and arteriovenous oxygen difference, with reduced oxygen delivery and unloading from both the fluorocarbon and plasma phase. Therefore, the efficacy of PFCOCs at 2,600 m above sea level is reduced, and altitude must be taken into account when PFCOCs are used.