Characterizing functional differences in sea anemone Hsp70 isoforms using budding yeast
Characterizing functional differences in sea anemone Hsp70 isoforms using budding yeast
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DOI：
10.1007/s12192-018-0900-7
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发表时间：
2018-04
影响因子：
3.8
通讯作者：
Shawn J. Waller;Laura E. Knighton;Lenora M. Crabtree;Abigail Perkins;A. Reitzel;A. Truman
中科院分区：
文献类型：
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作者：
Shawn J. Waller;Laura E. Knighton;Lenora M. Crabtree;Abigail Perkins;A. Reitzel;A. Truman
海洋生物会受到非生物的压力，如温度、紫外线辐射、盐度和氧气浓度的波动。热休克蛋白（HSP）通过重折叠和维持受损蛋白质的活性来帮助细胞对这些应激源做出反应。高度保守的Hsp70分子伴侣家族对于细胞活力以及对应激的反应是必需的。生物体具有多种Hsp70亚型，其氨基酸序列略有不同，但对其功能相关性知之甚少。在本研究中，我们对来自于小海葵Nematostella vectensis的三种主要Hsp70亚型NvHsp70 A、B和D进行了分析，并通过转录分析和在出芽酵母Saccharomyces cerevisiae中的异源表达来评估Hsp70亚型在小海葵中的功能。有趣的是，发现这些亚型不仅在应激下表达不同，而且在介导细胞应激程序的能力方面似乎具有功能差异。这些结果有助于了解热休克蛋白70亚型的特异性，其共同的和独特的作用，在应对急性和慢性的环境压力，和当地的适应在N的人口的潜在基础。vectensis。
Marine organisms experience abiotic stressors such as fluctuations in temperature, UV radiation, salinity, and oxygen concentration. Heat shock proteins (HSPs) assist in the response of cells to these stressors by refolding and maintaining the activity of damaged proteins. The well-conserved Hsp70 chaperone family is essential for cell viability as well as the response to stress. Organisms possess a variety of Hsp70 isoforms that differ slightly in amino acid sequence, yet very little is known about their functional relevance. In this study, we undertook analysis of three principal Hsp70 isoforms NvHsp70A, B, and D from the starlet sea anemoneNematostella vectensis.The functionality of Hsp70 isoforms in the starlet sea anemone was assessed through transcriptional analysis and by heterologous expression in budding yeastSaccharomyces cerevisiae. Interestingly, these isoforms were found to not only differ in expression under stress but also appear to have functional differences in their ability to mediate the cellular stress program. These results contribute to an understanding of Hsp70 isoform specificity, their shared and unique roles in response to acute and chronic environmental stress, and the potential basis of local adaptation in populations ofN. vectensis.