Effective chair training methods for neuroscience research involving rhesus macaques (Macaca mulatta)
Effective chair training methods for neuroscience research involving rhesus macaques (Macaca mulatta)
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DOI：
10.1016/j.jneumeth.2019.02.001
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发表时间：
2019-04-01
影响因子：
3
通讯作者：
Mitchell, Anna S.
中科院分区：
文献类型：
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作者：
Mason, Stuart;Premereur, Elsie;Mitchell, Anna S.
背景：使用猕猴的神经科学研究仍然是我们努力了解人类大脑功能的基础。应用3R的优化原则对于优化猴子的福利至关重要，并且仍然产生高质量的科学。新方法：在这里，我们记录了我们对46只雄性恒河猴进行运输设备（即灵长类椅子或运输箱）适应训练方法的改进。我们的训练方法一直使用正强化训练（PRT）。然而，PRT有时与负强化训练（NRT）相结合，但不杆和衣领技术，成功地将每只猴子从其家庭外壳到其运输device.Results和比较与现有的方法：训练猴子对或组，并开始他们的PRT训练后，抵达单位内减少了所需的天数来适应他们。虽然PRT的使用对于与猴建立积极的关系至关重要，但NRT技术有时也是必要的，并且在猴做出所需反应后立即撤回时最有效，以减少适应天数。一旦适应了他们的椅子，猴子成功地在10天内自愿提出他们的头部颈部板使用PRT。椅子内的空间减少也促进了一些monkeys.Conclusions头部presentation：驯化（塑造）的猴子运输设备可以是一个紧张的经验，猴子和教练。我们对训练的适应大大减少了在这个阶段花费的时间。我们认为这种天数的减少是对猴子神经科学研究中3R（改进）的有效实施。
Background: Neuroscience research using macaques remains fundamental in our endeavours to understand how the human brain functions. Applying the refinement principle of the 3Rs is essential to optimise the monkeys' welfare and still produces high quality science.New method: Here we document refinements in our training methods for acclimation to transport devices (i.e. primate chair or transport box) while working with 46 male rhesus macaques. Our training methods always used positive reinforcement training (PRT). However, PRT was sometimes combined with negative reinforcement training (NRT), but not pole and collar techniques, to successfully transfer each monkey from its home enclosure to its transport device.Results and comparison with existing methods: Training monkeys in pairs or groups, and starting their PRT training upon arrival within the unit reduced the days required to acclimate them. While the use of PRT is essential to establish a positive relationship with monkeys, NRT techniques are sometimes necessary, and are most effective when withdrawn immediately once the monkey makes the desired response, to reduce the days of acclimation. Once acclimatised to their chair, monkeys succeeded within 10 days to present their head voluntarily for neck plating using PRT. Space reducers inside the chairs also facilitated head presentations for some monkeys.Conclusions: Acclimating (shaping) the monkeys to transport devices can be a stressful experience for monkeys and trainers. The adaptations to our training substantially reduced the days spent on this stage. We view this reduction in days as an effective implementation of the 3Rs (refinement) in monkey neuroscience research.