Ultrasensitive multispecies spectroscopic breath analysis for real-time health monitoring and diagnostics
Ultrasensitive multispecies spectroscopic breath analysis for real-time health monitoring and diagnostics
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DOI：
10.1073/pnas.2105063118
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发表时间：
2021-10-05
影响因子：
11.1
通讯作者：
Toscano, Jutta
中科院分区：
文献类型：
--
作者：
Liang, Qizhong;Chan, Ya-Chu;Toscano, Jutta
呼吸分析通过识别和量化呼出的生物标志物，实现快速、非侵入性诊断以及长期监测人体健康。在这里，我们展示了显着的能力，中红外（中红外）腔增强的直接频率梳状光谱（CE-DFCS）应用于呼吸分析。我们同时检测和监测作为时间的函数四个呼吸生物标志物-CH 3OH，CH 4，H2O，和HDO-以及说明检测至少六个（H2 CO，C2 H6，OCS，C2 H4，CS2，和NH 3）的可行性，而无需修改的实验装置。我们实现了在万亿分之一水平上的超声波检测灵敏度。这是通过频率梳的宽带光谱覆盖范围、单个梳齿提供的高光谱分辨率以及高精细度腔体产生的灵敏度增强的组合而实现的。利用频率梳、光学涂层和光电探测器技术的最新进展，我们可以获得大量在中红外具有强碳氢键光谱特征的生物标志物。
Breath analysis enables rapid, noninvasive diagnostics, as well as long-term monitoring of human health, through the identification and quantification of exhaled biomarkers. Here, we demonstrate the remarkable capabilities of mid-infrared (mid-IR) cavity-enhanced direct-frequency comb spectroscopy (CE-DFCS) applied to breath analysis. We simultaneously detect and monitor as a function of time four breath biomarkers-CH3OH, CH4, H2O, and HDO-as well as illustrate the feasibility of detecting at least six more (H2CO, C2H6, OCS, C2H4, CS2, and NH3) without modifications to the experimental apparatus. We achieve ultrahigh detection sensitivity at the parts-per-trillion level. This is made possible by the combination of the broadband spectral coverage of a frequency comb, the high spectral resolution afforded by the individual comb teeth, and the sensitivity enhancement resulting from a high-finesse cavity. Exploiting recent advances in frequency comb, optical coating, and photodetector technologies, we can access a large variety of biomarkers with strong carbon-hydrogen-bond spectral signatures in the mid-IR.