The dynamics of postmitotic reassembly of the nucleolus.
The dynamics of postmitotic reassembly of the nucleolus.
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DOI：
10.1083/jcb.150.3.433
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发表时间：
2000-08-07
期刊：
影响因子：
--
通讯作者：
Olson MO
中科院分区：
文献类型：
--
作者：
Dundr M;Misteli T;Olson MO
哺乳动物细胞核仁在M期开始时解体，并在末期重新组装。最近的研究表明，在有丝分裂过程中，部分加工的前核糖体RNA（pre-rRNA）与染色体周围区域（PR）和称为核仁衍生灶（NDF）的颗粒中的加工组分一起保存。在这里，核仁重组的动力学进行了研究，首次在活细胞表达融合的加工相关蛋白原纤蛋白，核仁素，或B23与绿色荧光蛋白（GFP）。在末期的NDF消失的同时出现的材料在重整核。前核仁体（PNB）在末期早期出现在细胞核中，随着核仁的形成而逐渐消失，强烈表明PNB组分转移到新形成的核仁中。光漂白后的荧光恢复（FRAP）表明，原纤蛋白-GFP与NDF和PNB以快速和相似的速率重新结合。PNBs中存在前rRNA序列，表明加工复合物重新进入末期细胞核。特定蛋白质进入核仁与加工事件的时间大致相关。有丝分裂保存的加工复合物可能是必不可少的调节组件的分布，重新组装子细胞核仁。
Mammalian cell nucleoli disassemble at the onset of M-phase and reassemble during telophase. Recent studies showed that partially processed preribosomal RNA (pre-rRNA) is preserved in association with processing components in the perichromosomal regions (PRs) and in particles called nucleolus-derived foci (NDF) during mitosis. Here, the dynamics of nucleolar reassembly were examined for the first time in living cells expressing fusions of the processing-related proteins fibrillarin, nucleolin, or B23 with green fluorescent protein (GFP). During telophase the NDF disappeared with a concomitant appearance of material in the reforming nuclei. Prenucleolar bodies (PNBs) appeared in nuclei in early telophase and gradually disappeared as nucleoli formed, strongly suggesting the transfer of PNB components to newly forming nucleoli. Fluorescence recovery after photobleaching (FRAP) showed that fibrillarin-GFP reassociates with the NDF and PNBs at rapid and similar rates. The reentry of processing complexes into telophase nuclei is suggested by the presence of pre-rRNA sequences in PNBs. Entry of specific proteins into the nucleolus approximately correlated with the timing of processing events. The mitotically preserved processing complexes may be essential for regulating the distribution of components to reassembling daughter cell nucleoli.
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影响因子：
3.3
作者：
JIMENEZGARCIA, LF;SEGURAVALDEZ, MD;SPECTOR, DL
通讯作者：
SPECTOR, DL
DOI：
10.1083/jcb.118.6.1297
发表时间：
1992-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bell P;Dabauvalle MC;Scheer U
通讯作者：
Scheer U
影响因子：
3.7
作者：
Fomproix, N;Hernandez-Verdun, D
通讯作者：
Hernandez-Verdun, D
DOI：
10.1073/pnas.88.3.931
发表时间：
1991-02-01
影响因子：
11.1
作者：
ARIS, JP;BLOBEL, G
通讯作者：
BLOBEL, G
影响因子：
3.7
作者：
MEDINA, FJ;CERDIDO, A;FERNANDEZGOMEZ, ME
通讯作者：
FERNANDEZGOMEZ, ME