Effect of CD133/prominin-1 antisense oligodeoxynucleotide on in vitro growth characteristics of Huh-7 human hepatocarcinoma cells and U251 human glioma cells.
Effect of CD133/prominin-1 antisense oligodeoxynucleotide on in vitro growth characteristics of Huh-7 human hepatocarcinoma cells and U251 human glioma cells.
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DOI：
10.3892/or_00000500
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发表时间：
2009-10
期刊：
影响因子：
4.2
通讯作者：
Jie Yao;Zhang Tao;Jinghua Ren;M. Yu;Gang Wu
中科院分区：
文献类型：
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作者：
Jie Yao;Zhang Tao;Jinghua Ren;M. Yu;Gang Wu
干细胞标记物CD133/重要蛋白-1是一种五聚糖膜糖蛋白，在前列腺癌、肝脏、结肠和脑等多种实体肿瘤中也被鉴定为肿瘤干细胞(CSC)标记物。CD133在这些肿瘤中的作用尚不清楚。我们推测CD133的功能与CD133+CSCs的特性有关，并可能影响整个肿瘤细胞群体的生长。在本研究中，我们使用CD133的反义寡核苷酸(ASODN)来下调CD133的表达。我们发现CD133-KD抑制了U251人胶质瘤细胞的增殖，降低了HUH-7人肝癌细胞的集落形成能力，改变了细胞周期分布。我们的数据表明，CD133可能在这些肿瘤细胞的生长中发挥重要的功能作用。此外，这项研究还表明，与CD133/AR-1属于同一家族的另一种蛋白-2可能没有类似的功能。
The stem cell marker CD133/prominin-1, is a pentaspan membrane glycoprotein, which has also been identified as a cancer stem cell (CSC) marker in several solid tumor types, including those of the prostate, liver, colon and brain. The function of CD133 in these tumors is still unknown. We hypothesize that the function of CD133 is correlated with the characteristics of the CD133+ CSCs and may affect the growth of the tumor cell population as a whole. In this study we used antisense oligodeoxynucleotides (ASODNs) of CD133 to knock-down (KD) CD133 expression. We showed that the CD133-KD inhibited proliferation of U251 human glioma cells, decreased the colony forming ability and altered the cell cycle distribution in Huh-7 human hepatocellular carcinoma cells. Our data suggest that CD133 may play an important functional role in the growth of these tumor cells. Moreover, this study also showed that prominin-2, another protein in the same family as CD133/prominin-1, may not have a similar function.